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Plasmid
pFBDM6P1b 6His TEV HA xenopus fancd2/V5 xenopus fanci
Parent Plasmid
pFBDM6P1b
Expressed
V5 xenopus fanci
DU Number
DU53172
Genbank
NM_001171211.1 GI:284005540
Species
Xenopus laevis
Sequence of Insert
View Sequence
CTCGAGGAAACCATGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGATGGACCAAAAAATTCTGTCTTTGGCTGCTGAAGAACAAAATGATGGACTTCAGAGTTATCTTCAAAACCTGAAAGAAACCGAGCTTTCAGAAATCATAACAAAGCATGCAGTTAAGGGAAAAGACTGTGGAGCGCTATTGCGAGGCGTCTTTAAAGGGTCTCCGTGCTCTCATGATGTAGCCGTACGCCGAAGGCTCGCGGTTTATAGACACTGCATACAGCTTGTTGAGTCCGGGGACTTGCAACGAGAGGTTGCATCTGAGATTATGGGTTTATTAATGCTGGAGGTCCATCATTTTCCGGGTGCTTCGCTCATTGAACTGGCTAACTTATTTGCGGATGCTGTTAAAGCTGGTACTCTGTCCAATGGAAAATCTTTAGATTTGTTTCCCACAGTACTTACTGCGCTTTCTTCAAAAGAAAGCTTAGCATATGGTAAAGGTGAACTTACTGGAGAGGAATTTAAGAAACAACTTATAAATAGCCTTTGTTCTAGCAGATGGGATCCACAGAGTGTGATTTTTCTTACTTCAATGTTTAGGGATGTTTCCTTGAGTGCCGAAGAGCTGCAGTTTGTTGTGGAAAAGGTTTTAAGGATGCTTTCTAAGTTAGACCTCCAGGAAATGCCCCCCTTAGTTTACCAATTGCTACTCCTGTCTGCTAAGGGTAGTAAAAAGAATATTGTGGAAGGAATTATCACTATATTTAATGATCTTGATCAGAAGCAGTTAGTACAGCAAGAGAACTCTGAATCATTAGACTTGGAAGATGCAACCATCCCACAAGACCAGCTGCACCACGTGGAGGGCACAATCATTTTACACATCGTTTTTGCAATTAAGTTGGACCAGGAATTAGGAAGAGAGCTTCTGAAGTATCTCAAGGCTGGCCAACAAGGGGACTCAAGCAAGATCCTTTGCCCTTTCAGTGCTGCCCTCTTGTTATCTGTATCAAGGATTCATAGATTCCAAGAACAGGTATTTGATTTTCTCAAATCTACTATACTGAGAGGTTATAAAGACCTTCAGTTTCATCAAAGCTCAAAATTCCTTCAGGACTTAGTTCCCCAGCCTGTATGCGTATCTTCAATTCTTCTGGAAACTGTTAAAAACAGTGTTTATGGATGGGACCATGTCACTCAAGGGCTTGTGGAACTTGGTTTTATTTTAATGGATTCTTTTGGACCCAAAGCAGCCTTTGGAAATAAAGTGGTTGAGCTTATTTCCGTGCATTCCAGGACACCTTCCCAGCAAGCTTGTCAGCTTGGATCTAGAATCTTGCTAGAAACTTTTAAGGTCCATGAGCCTATCAGAAGTGAAATTCTGGAGCAGGTCTTGAATCGTGTTATCACAAAAGCTGCAACTCCAGTTACACATTTTATAGATCTTTTATCAGATATTGTGGTTTCTGCTCCGCTCATCCTTCAGAATTCATCATCTAAAGTTACTGAAGCATTTGACCACTTGTCCTTCCTGCCTCTTACAACTGTTCAGGGGCTCCTGAAAGCTGTGCAGCCCCTTCTTAAAATCAGTATGTCCATGAGGGATTCCCTTATTCTTGTTCTAAGAAAAGCCATGTTCTCAAGTCAAATAGATGCAAGAAAGTCGGCTGTCGCTGGGTTTCTTCTCCTACTGAGGAACTTTAAAGTCTTAGGAAGTCTGTCCTCATCTCAGTGCAGCCAAGCTATTGGTGCTAGCCAGATCCAGGTGGATGTCCATATGCGTTACAATGCTGCAGCAAATGAGGCCTTTTGTCTGGAAATCCTTGGCAGTCTGAGGAGGTGCTTAAGCCAGCAAGCAGATGTTAGGCTCATGCTTTACGAGGGTTTCTATGATGTCCTGCGACGAAATTCCCAGCTTGCCAGTTCTGTCATGCAGACTCTGTTGTCTCAGTTAAAGCGATATTATGAACCCGAACCTGATCTTTTGCCACCATTGAAGTTGGAGGGCTGTATTACTGCTCAAGGGGATCATATATTTTTGCAGGAACCTCTGGCTCACCTGTTGTGCTGTATCCACCATTGCTTGCAGTGGTATAAGAGCAGTTTACAGCAGCATAGGAATCCTGATGAAGATGATGACGATGATGACCAAACGGGATGTCAACAGGACCTTAATGATATAATGGAGTCCATCACAAGGAGAATGATTAAATGTGACCTGGAGGACTTTGAATTAGACAAGTCTGCAGACTTTTCTTTGGCGTCTGGTGTTGGAGTGAAAAATAACATATATGCTGTTTTGGTAATGGGGATTTGTGAAGTCCTAATAGAATACAACTTTATCATTGCAAATTTCAGCAAGAGCAAATTTGAGGACATTTTGGGCCTGTTTAAGTGCTACAGCAAACTGTCCGATATCTTAAAGGAGAAAGCTGCTAAAGGCAGACAGCCTGGGAACAATAAAACGGCCAGAAGCCTCATATCAATGACTTTTGTTTCTACACTCTTAACTGCTTTATTCAGAGACAGCACACGCTCGCACGAGGAGAGTCTTTCTATTCTTCGCGCAAATGTGGATTTCATGCGCTATTCGGTGTGTGTTGCCCTTCAAAAAATTCAGCAGCTTGAAGAAACTGGTGTCACAGATGGACCTGATGGACAGAACAGCGAGAAAATGTTCCGAAGTCTATGTGAAATTACCAGGGTACTTATGTGGAGATACACCTCAATTCCAGCTGCAGCTGAGAATCCTGGGAAGAAGGACAAAGGCAAAACCATTTCCTTGCTGTGCCTGGAGGGATTGCTGAGAGTGTTTAACACCGTTCAGCAACGATATCCATCAAAGATCCCACAATTCCTTACTGCGTTGGATGCCCTAGGGGATGAGGATGAAGAAGGCTCAAGAGAAATCAATGTGACCGAGAAGGCAGCTTTTCAGATTAAACAGTTTCAAAGATCTCTTATTAACCAACTCAGTGGTGGAGAGGATGACTTTAACAGCAAAGAAGCTCTACTGTTAGTCAGCATCTTGTCTACATTATCCAGGTTACTGGCCCCCTCTTCTCAACAGTTCGTGCAGATGCTGTCTTGGACTGTTAAAATCTGTAAAGAGACAAACATTGAGGATGTCCAGTTTTGCAAGGGAATAATGAACCTTCTCTTTAGCCTCCATGTTCAGTTCAAGAGCCCAGTGAGTGTGCTTCGAGAACTGTGCCAGGACATCCATGGGCATCTGGGTGATATTGATCAGGATATTGAAGTGGAGAAGCAGTCTCATTTTGCTACTGTAAGTCTCAAGACTGTAGCCCCGACTGTTACTTTGTTGGTTCTGGGTCAAGCTGCAAAGGTCCTGGAAGAAGTGGACTGGCTGATCATAAAGCTGAAAGGACTACTGGGTTCAGAGAAGCTCTCTACGGAAGATTTAACTCAGACTTCAAATGCAAGGGTTCCAATAGAAAAAGCTATCATTCTTCAGTTGGGCACACTGCTGACTGCCTGCCATGAGTTAGTGCAGACTGCTCTACCTGCTGGGAGTTGTACTGACACTCTTCTGAAGGAGCTGGCAAAGATGTACACCATCCTCACGTCCGTTGTCAAATACTACTTACAGATCTGCAGCAGTCACGGGGGACAGATTTCTGCTCGGTTGGAGAAGCTGGTGAAGCTTTCGGGTTCTCATCTTACCCCACAATGTTATGCCTTCATCACATATGTACAGAACATCCACAGTGAAAGTCTAAGCCTTGCTGCAGAAAAGAAGAAGAAAAAGAAGGAAGAGGCATTGACTGCCACCTCAGCCAAAATCCTGCGTGATACTAAGCCCATACCAAACCTGATCTTTGCCATTGAACAGTATGAGAAGTTTCTCATTCATCTCTCCAAGAAGTCCAAGGTGAACTTGATGCAATACATGAAGCTGAGCACCTCAAGGGACTTCAGGATAAATGCAGCCACACTAGACGCAGCTTTACAAGAGAAAGGGTCTGAAGATGAAGAGAATGAGCCAGATAATGAACAGGCTGTAACAGAAGAAGAAAGTCAAGAACCAAAGAAGAAGAGGCGCAGAAAATAACTCGAG
Amino Acid Sequence
View Sequence
MGKPIPNPLLGLDSTMDQKILSLAAEEQNDGLQSYLQNLKETELSEIITKHAVKGKDCGALLRGVFKGSPCSHDVAVRRRLAVYRHCIQLVESGDLQREV
ASEIMGLLMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKESLAYGKGELTGEEFKKQLINSLCSSRWDPQSVIFLTSMFRDVSL
SAEELQFVVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQKQLVQQENSESLDLEDATIPQDQLHHVEGTIILHIVFAIKLDQE
LGRELLKYLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDLQFHQSSKFLQDLVPQPVCVSSILLETVKNSVYGWDHVTQGLVE
LGFILMDSFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEILEQVLNRVITKAATPVTHFIDLLSDIVVSAPLILQNSSSKVTEA
FDHLSFLPLTTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFSSQIDARKSAVAGFLLLLRNFKVLGSLSSSQCSQAIGASQIQVDVHMRYNAAANEAFC
LEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDLLPPLKLEGCITAQGDHIFLQEPLAHLLCCIHHCLQWYKSSLQQ
HRNPDEDDDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKNNIYAVLVMGICEVLIEYNFIIANFSKSKFEDILGLFKCYSKLS
DILKEKAAKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFMRYSVCVALQKIQQLEETGVTDGPDGQNSEKMFRSLCEITRVLM
WRYTSIPAAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGDEDEEGSREINVTEKAAFQIKQFQRSLINQLSGGEDDFNSKEAL
LLVSILSTLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFKSPVSVLRELCQDIHGHLGDIDQDIEVEKQSHFATVSLKTVAPT
VTLLVLGQAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGTLLTACHELVQTALPAGSCTDTLLKELAKMYTILTSVVKYYLQI
CSSHGGQISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEALTATSAKILRDTKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQYM
KLSTSRDFRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*
ASEIMGLLMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKESLAYGKGELTGEEFKKQLINSLCSSRWDPQSVIFLTSMFRDVSL
SAEELQFVVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQKQLVQQENSESLDLEDATIPQDQLHHVEGTIILHIVFAIKLDQE
LGRELLKYLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDLQFHQSSKFLQDLVPQPVCVSSILLETVKNSVYGWDHVTQGLVE
LGFILMDSFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEILEQVLNRVITKAATPVTHFIDLLSDIVVSAPLILQNSSSKVTEA
FDHLSFLPLTTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFSSQIDARKSAVAGFLLLLRNFKVLGSLSSSQCSQAIGASQIQVDVHMRYNAAANEAFC
LEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDLLPPLKLEGCITAQGDHIFLQEPLAHLLCCIHHCLQWYKSSLQQ
HRNPDEDDDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKNNIYAVLVMGICEVLIEYNFIIANFSKSKFEDILGLFKCYSKLS
DILKEKAAKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFMRYSVCVALQKIQQLEETGVTDGPDGQNSEKMFRSLCEITRVLM
WRYTSIPAAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGDEDEEGSREINVTEKAAFQIKQFQRSLINQLSGGEDDFNSKEAL
LLVSILSTLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFKSPVSVLRELCQDIHGHLGDIDQDIEVEKQSHFATVSLKTVAPT
VTLLVLGQAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGTLLTACHELVQTALPAGSCTDTLLKELAKMYTILTSVVKYYLQI
CSSHGGQISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEALTATSAKILRDTKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQYM
KLSTSRDFRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Fancy changes to NM_001171211.1 GI:284005540 in this clone: a1013g (D338G), g2342a (C781Y), c3245t (A1082V), t3731c (V1244A) and silent change c3753t. Derive from clone provided by Mark Frost (Helen Walden group).
N.B. Repropagate at 30 degrees.