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Plasmid
pET28a-6His-HALO-C3-4Gly-UbcH7 C17S C137S
Parent Plasmid
pET28a-6His-HALO-C3
Expressed
6His-HALO-C3-4Gly-UbcH7 C17S C137S
DU Number
DU28242
Genbank
P68036
Tag
6His
HALO
Species
Human
Sequence of Insert
View Sequence
GGATCCGGTGGCGGGGGTATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATCTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTCTAAGAATGCTGAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGLEVLFQGPGSGGGGMAASRRLMKELEEIRKSGMKNFRNIQVDEANL
LTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEK
GQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKD
RKKFSKNAEEFTKKYGEKRPVD*
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGLEVLFQGPGSGGGGMAASRRLMKELEEIRKSGMKNFRNIQVDEANL
LTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEK
GQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKD
RKKFSKNAEEFTKKYGEKRPVD*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Parental 28224
100042 R 1000 1001 1047 19007
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