This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Synonyms
FAAP43; PHF9; POG
Plasmid
pFastbac1 6His TEV codon optimised mouse FancI M1-E1301 13TGS(MG) codon optimised FANCL tv2 Q109-E294
Parent Plasmid
pFastbac1b
Expressed
6His TEV codon optimised mouse FancI M1-E1301 13TGS(MG) codon optimised FANCL tv2 Q109-E294
DU Number
DU53029
Genbank
mouse FancI:codon optimised NM_145946.2 Human FANCL: codon optimised NM_018062.3 GI:167860136
Tag
6His
Sequence of Insert
View Sequence
CCATGGGCAGCAGCCATCACCATCACCATCACGAAAACCTGTATTTTCAGATGGACCTGAAGATCCTGTCCCTGGCTACCGACAAGACCACCGACAAGCTGCAAGAGTTCCTGCAGACCCTGAAGGACGACGACCTGGCTTCCCTGCTGCAGAACCAGGCTGTGAAGGGTCGTGCTGTGGGCACCCTGCTGCGTGCTGTGCTGAAGGGTTCCCCCTGCTCCGAAGAGGACGGTGCTCTGCGCCGTTACAAGATCTACTCCTGCTGCATCCAGCTGGTCGAGTCCGGCGACCTCCAGCAGGATGTGGCTTCCGAGATCATCGGCCTGCTGATGCTCGAGGTGCACCACTTCCCTGGTCCTCTGCTGGTGGACCTGGCCTCCGATTTCGTGGGTGCTGTGCGCGAGGACCGTCTGGTCAACGGCAAGTCCCTGGAACTGCTGCCCATCATCCTGACCGCTCTGGCTACCAAGAAGGAAGTCCTGGCTTGCGGCAAGGGCGACCTGAACGGCGAAGAGTACAAGCGTCAGCTGATCGACACCCTGTGCTCCGTGCGTTGGCCCCAGCGTTACATGATCCAGCTGACCTCCGTGTTCAAGGACGTGTGCCTGACCCCCGAAGAGATGAACCTGGTGGTGGCTAAGGTGCTGACCATGTTCTCCAAGCTGAACCTGCAAGAGATCCCCCCACTGGTGTACCAGCTGCTGGTCCTGTCCTCCAAGGGCTCCCGTCGTTCTGTGCTGGACGGTATCATCGCTTTCTTCCGTGAACTGGACAAGCAGCACCGCGAGGAACAGTCCTCCGACGAGCTGTCCGAGCTGATCACCGCTCCTGCTGACGAACTGTACCACGTCGAGGGCACCGTGATCCTGCACATCGTGTTCGCTATCAAGCTGGACTGCGAGCTGGGTCGCGAGCTGCTGAAGCACCTGAAGGCTGGTCAACAGGGCGACCCCTCCAAGTGCCTGTGCCCCTTCTCTATCGCTCTGCTGCTGTCCCTGACCCGTATCCAGCGTTTCGAAGAACAGGTGTTCGACCTGCTCAAGACCTCCGTCGTGAAGTCCTTCAAGGACCTGCAGCTCCTGCAGGGTTCCAAGTTCCTCCAGACTCTGGTGCCCCAGAGGACCTGCGTGTCCACCATGATCCTGGAAGTCGTGCGCAACTCCGTGCACTCCTGGGACCACGTGACCCAGGGCCTGATCGAGTTCGGTTTCATCCTGATGGACTCCTACGGTCCCAAGAAGATCCTCGACGGCAAGGCTGTCGAGATCGGCACCTCCCTGTCCAAGATGACCAACCAGCACGCCTGCAAGCTGGGTGCTAACATCCTCCTGGAAACCTTCAAGATCCACGAGATGATCCGCCAAGAAATCCTGGAACAGGTGCTGAACCGTGTCGTGACCCGTACCTCCTCCCCTATCAACCACTTCCTGGACCTGTTCTCCGACATCATTATGTACGCTCCTCTGATCCTCCAGAACTGCTCCAAAGTGACCGAGACTTTCGACTACCTGACCTTCCTGCCTCTGCAGACCGTGCAGGGACTGCTGAAGGCCGTGCAGCCCCTGCTGAAGATCTCCATGTCCATGCGCGACTCCCTGATCCTGGTGCTGCGCAAGGCTATGTTCGCTTCCCAGCTGGACGCTCGCAAGTCCGCTGTGGCTGGTTTCCTGCTGCTCCTGAAGAACTTCAAGGTGCTGGGTTCCCTGCCATCCTCCCAGTGCACCCAGTCCATCGGTGTCACCCAAGTGCGCGTGGACGTGCACTCTCGTTACTCCGCTGTCGCTAACGAAACCTTCTGCCTGGAAATCATCGACTCCCTCAAGCGTTCCCTGGGCCAGCAGGCTGACATCCGTCTGATGCTGTACGACGGTTTCTACGACGTGCTGCGTCGTAACTCTCAGCTGGCTTCCTCCATCATGCAGACTCTGTTCTCCCAGCTCAAGCAGTTCTACGAGCCCGAGCCCGACCTGCTGCCTCCTCTGAAGTTGGGAGCTTGCGTGCTGACTCAGGGTTCCCAGATCTTCCTGCAAGAACCCCTGGACCACCTCCTGTCCTGCATCCAACACTGCCTGGCTTGGTACAAGTCCCGTGTGGTGCCCCTGCAGCAAGGCGACGAGGGCGAGGAAGAGGAAGAAGAACTGTACTCCGAGCTGGACGACATGCTGGAATCCATCACCGTGCGTATGATCAAGTCCGAGTTGGAGGACTTCGAGTTGGACAAGTCCGCCGACTTCTCCCAGAACACCAACGTGGGCATCAAGAACAACATCTGCGCTTGCCTGATCATGGGCGTGTGCGAGGTGCTGATGGAGTACAACTTCTCCATCTCGAACTTCAGCAAGTCCAAGTTCGAAGAGATCCTGAGCCTGTTCACTTGCTACAAGAAGTTCAGCGACATCCTGTCCGAGAAGGCTGGCAAGGGCAAGGCTAAGATGACCTCCAAGGTGTCCGACTCTCTGCTGTCTCTCAAGTTCGTGTCTGACCTGCTGACCGCCCTGTTCCGTGACTCCATCCAGTCCCACGAGGAATCCCTGTCCGTGCTGAGATCCTCCGGCGAGTTCATGCACTACGCTGTGAACGTGACCCTCCAGAAGATCCAGCAGCTCATCCGTACCGGTCACGTGTCCGGACCTGACGGCCAGAACCCCGACAAGATCTTCCAGAACCTGTGCGACATCACCCGTGTGCTGCTGTGGCGTTACACCTCCATCCCCACCTCCGTGGAAGAGTCCGGAAAGAAGGAAAAGGGCAAGTCCATCTCCCTGCTGTGCCTGGAAGGCCTGCAAAAGACCTTCTCCGTGGTGCTCCAGTTCTACCAACCCAAGGTGCAGCAATTCCTGCAGGCTCTGGACGTGATGGGTACTGAAGAGGAAGAGGCTGGCGTCACCGTGACCCAGAGGGCTAGCTTCCAGATCCGTCAGTTCCAGCGTTCTCTGCTCAACCTGCTGTCCAGCGAAGAGGATGACTTCAACTCCAAGGAAGCCCTGCTGCTGATCGCCGTGCTGTCCACCCTGTCCCGTCTGCTGGAACCTACCTCCCCACAGTTCGTGCAGATGCTGTCCTGGACTTCCAAGATCTGCAAGGAATACTCTCAAGAGGACGCCAGCTTCTGCAAGTCTCTGATGAACCTCTTCTTCTCCCTCCACGTGCTGTACAAGTCTCCCGTGACCCTGCTGAGGGACCTGTCCCAGGACATCCACGGTCAACTGGGAGACATCGACCAGGACGTGGAAATCGAAAAGACCGACCACTTCGCTGTGGTCAACCTGCGTACCGCTGCTCCCACTGTGTGCCTGCTGGTGTTGTCCCAGGCCGAGAAGGTCCTGGAAGAGGTCGACTGGCTGATCGCTAAGATCAAGGGTTCCGCTAACCAGGAAACCCTGTCTGACAAAGTGACTCCCGAGGACGCCTCCTCCCAGGCTGTTCCTCCTACCCTGCTCATCGAGAAGGCCATCGTGATGCAGCTGGGTACTCTGGTCACCTTCTTCCACGAACTGGTGCAGACTGCTCTGCCCTCCGGTTCCTGCGTGGACACCCTCCTGAAGGGCCTGTCTAAGATCTACAGCACCCTGACCGCCTTCGTGAAGTACTACCTGCAAGTGTGCCAGTCCTCCCGTGGTATCCCCAACACCGTCGAGAAGCTGGTCAAGCTGTCCGGTTCCCACCTGACCCCTGTGTGCTACTCCTTCATCTCCTACGTGCAGAACAAGTCCTCTGACGCTCCCAAGTGCAGCGAAAAGGAAAAGGCTGCTGTCTCCACCACAATGGCTAAGGTCCTGCGCGAGACTAAGCCCATCCCCAACCTGGTGTTCGCCATCGAGCAGTACGAGAAGTTCCTGATCCAGTTGTCCAAGAAGTCCAAAGTCAACCTGATGCAGCACATGAAGCTGTCCACTTCCCGCGATTTCAAGATCAAGGGCAGCGTGCTGGACATGGTGCTCCGCGAGGATGAGACTGGATCGACCGGTTCAACCGGATCCACCGGGAGCACAGGCTCTACGGGATCGACTGGATCGACCGGTTCAACGGGGAGCACTGGTTCAACAGGTTCCACCGGAAGCACAGGAAGTATGGGACAGTTTTATAGCAGCCTGATTGAAGAAATTGGCACCCTGGGCTGGGATAAACTGGTGTATGCGGATACCTGCTTTAGCACCATTAAACTGAAAGCGGAAGATGCGAGCGGCCGTGAACATCTGATTACCCTGAAACTGAAAGCCAAATATCCGGCGGAAAGCCCGGATTATTTTGTGGATTTTCCGGTGCCGTTTTGCGCGAGCTGGACCCCGCAGAGCAGCCTGATCAGCATTTATAGCCAGTTTCTGGCCGCGATTGAAAGCCTGAAAGCGTTTTGGGATGTGATGGATGAAATCGATGAAAAAACCTGGGTGCTGGAACCGGAAAAACCGCCGCGTAGCGCGACCGCGCGTCGTATTGCGCTGGGCAACAACGTGAGCATTAACATTGAAGTGGACCCGCGTCATCCGACCATGCTGCCGGAATGCTTTTTTCTGGGCGCGGATCATGTGGTGAAACCGCTGGGCATTAAACTGAGCCGTAACATTCATCTGTGGGACCCGGAAAACAGCGTGCTGCAGAACCTGAAAGATGTGCTGGAAATTGATTTTCCGGCGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGSSHHHHHHENLYFQMDLKILSLATDKTTDKLQEFLQTLKDDDLASLLQNQAVKGRAVGTLLRAVLKGSPCSEEDGALRRYKIYSCCIQLVESGDLQQD
VASEIIGLLMLEVHHFPGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDV
CLTPEEMNLVVAKVLTMFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCE
LGRELLKHLKAGQQGDPSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIE
FGFILMDSYGPKKILDGKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETF
DYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCL
EIIDSLKRSLGQQADIRLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPL
QQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDIL
SEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRY
TSIPTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAV
LSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLV
LSQAEKVLEEVDWLIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQV
CQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSR
DFKIKGSVLDMVLREDETGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLI
TLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPE
CFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEIDFPA*
VASEIIGLLMLEVHHFPGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDV
CLTPEEMNLVVAKVLTMFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCE
LGRELLKHLKAGQQGDPSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIE
FGFILMDSYGPKKILDGKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETF
DYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCL
EIIDSLKRSLGQQADIRLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPL
QQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDIL
SEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRY
TSIPTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAV
LSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLV
LSQAEKVLEEVDWLIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQV
CQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSR
DFKIKGSVLDMVLREDETGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLI
TLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPE
CFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEIDFPA*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
N.B. Propagate at 30 degrees
100042 R 1000 1001 1047 19007
Download datasheet