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Plasmid
pFastbac1 6His TEV V5 Xenopus FancI K524R (with mutations - see notes)
Parent Plasmid
pFastbac1
Expressed
6His TEV V5 Xenopus FancI K524R (with mutations - see notes)
DU Number
DU50989
Genbank
NM_001171211.1 GI:284005540*
Tag
FLAG
Species
Xenopus laevis
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQGKPIPNPLLGLDSTMDQKILSLAAEEQNDGLQSYLQNLKETELSEIITKHAVKGKDCGALLRGVFKGSPCSHDVAVRRRLAVYRHCI
QLVESGDLQREVASEIMGLLMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKESLAYGKGELTGEEFKKQLINSLCSSRWDPQSV
IFLTSMFRDVSLSAEELQFVVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQKQLVQQENSESLDLEDATIPQDQLHHVEGTII
LHIVFAIKLDQELGRELLKYLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDLQFHQSSKFLQDLVPQPVCVSSILLETVKNSV
YGWDHVTQGLVELGFILMDSFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEILEQVLNRVITKAATPVTHFIDLLSDIVVSAPL
ILQNSSSKVTEAFDHLSFLPLTTVQGLLKAVQPLLKISMSMRDSLILVLRRAMFSSQIDARKSAVAGFLLLLRNFKVLGSLSSSQCSQAIGASQIQVDVH
MRYNAAANEAFCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDLLPPLKLEGCITAQGDHIFLQEPLAHLLCCIH
HCLQWYKSSLQQHRNPDEDDDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKNNIYAVLVMGICEVLIEYNFIIANFSKSKFED
ILGLFKCYSKLSDILKEKAAKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFMRYSVCVALQKIQQLEETGVTDGPDGQNSEKM
FRSLCEITRVLMWRYTSIPAAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGDEDEEGSREINVTEKAAFQIKQFQRSLINQLS
GGEDDFNSKEALLLVSILSTLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFKSPVSVLRELCQDIHGHLGDIDQDIEVEKQSH
FATVSLKTVAPTVTLLVLGQAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGTLLTACHELVQTALPAGSCTDTLLKELAKMYT
ILTSVVKYYLQICSSHGGQISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEALTATSAKILRDTKPIPNLIFAIEQYEKFLIHL
SKKSKVNLMQYMKLSTSRDFRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*
QLVESGDLQREVASEIMGLLMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKESLAYGKGELTGEEFKKQLINSLCSSRWDPQSV
IFLTSMFRDVSLSAEELQFVVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQKQLVQQENSESLDLEDATIPQDQLHHVEGTII
LHIVFAIKLDQELGRELLKYLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDLQFHQSSKFLQDLVPQPVCVSSILLETVKNSV
YGWDHVTQGLVELGFILMDSFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEILEQVLNRVITKAATPVTHFIDLLSDIVVSAPL
ILQNSSSKVTEAFDHLSFLPLTTVQGLLKAVQPLLKISMSMRDSLILVLRRAMFSSQIDARKSAVAGFLLLLRNFKVLGSLSSSQCSQAIGASQIQVDVH
MRYNAAANEAFCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDLLPPLKLEGCITAQGDHIFLQEPLAHLLCCIH
HCLQWYKSSLQQHRNPDEDDDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKNNIYAVLVMGICEVLIEYNFIIANFSKSKFED
ILGLFKCYSKLSDILKEKAAKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFMRYSVCVALQKIQQLEETGVTDGPDGQNSEKM
FRSLCEITRVLMWRYTSIPAAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGDEDEEGSREINVTEKAAFQIKQFQRSLINQLS
GGEDDFNSKEALLLVSILSTLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFKSPVSVLRELCQDIHGHLGDIDQDIEVEKQSH
FATVSLKTVAPTVTLLVLGQAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGTLLTACHELVQTALPAGSCTDTLLKELAKMYT
ILTSVVKYYLQICSSHGGQISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEALTATSAKILRDTKPIPNLIFAIEQYEKFLIHL
SKKSKVNLMQYMKLSTSRDFRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
a1013g (D338G), g2342a (C781Y), c3245t (A1082V), t3731c (V1244A) and silent change c3753t.
Derived from clone provided by Mark Frost (Helen Walden group).