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Plasmid
pET15 6His smt3 codon optimised FANCL tv2 Q109-H375-Linker-UBE2T
Parent Plasmid
pET15 6His thr smt3
Expressed
6His smt3 codon optimised FANCL tv2 Q109-H375-Linker-UBE2T
DU Number
DU50950
Genbank
See Notes
Tag
6His
SUMO
Species
Human
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLVPRGSHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPED
LDMEDNDIIEAHREQIGGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQF
LAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEI
DFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKHTGSTGSTGETGYTQG
MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRP
SLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV*
LDMEDNDIIEAHREQIGGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQF
LAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEI
DFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKHTGSTGSTGETGYTQG
MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRP
SLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV*
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
FANCL: codon optimised NP_001108108.1
UBE2T: NM_014176.3 GI:209969667
FANCL fragment cloned in BglII/KpnI, followed by Linker-UBE2T into NotI and removal of extra residue after smt3 removed by mutagenesis