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Plasmid
pcDNA5D FRT/TO BirA R118G-USP49 C262A-HA
Parent Plasmid
pcDNA FRT TO BirA-MCS-HA
Expressed
BirA R118G-USP49 C62A-HA
DU Number
DU28148
Tag
HA
BirA
Species
Human
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMDRCKHVGRLRLAQDHSILNPQKWCCL
ECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCY
LCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVV
LPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEA
LERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPA
ALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTAYMNSILQVLSHLQKF
RECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACER
EGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVS
PFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIP
FSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPE
RYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSN
PKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVL
TMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGA
CALLCGVGDTERGAAAYPYDVPDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMDRCKHVGRLRLAQDHSILNPQKWCCL
ECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCY
LCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVV
LPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEA
LERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPA
ALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTAYMNSILQVLSHLQKF
RECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACER
EGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVS
PFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIP
FSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPE
RYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSN
PKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVL
TMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGA
CALLCGVGDTERGAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 49108.
100042 R 1000 1001 1047 19007
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