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Plasmid
pcDNA5D FRT/TO BirA R118G-USP49-HA
Parent Plasmid
pcDNA FRT TO BirA-MCS-HA
Expressed
BirA R118G-USP49-HA
DU Number
DU28147
Tag
HA
BirA
Species
Human
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMDRCKHVGRLRLAQDHSILNPQKWCCL
ECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCY
LCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVV
LPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEA
LERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPA
ALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKF
RECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACER
EGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVS
PFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIP
FSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPE
RYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSN
PKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVL
TMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGA
CALLCGVGDTERGAAAYPYDVPDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMDRCKHVGRLRLAQDHSILNPQKWCCL
ECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCY
LCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVV
LPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEA
LERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPA
ALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKF
RECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACER
EGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVS
PFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIP
FSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPE
RYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSN
PKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVL
TMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGA
CALLCGVGDTERGAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 49108.
100042 R 1000 1001 1047 19007
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