This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1
Plasmid
pCAGGS puro HA Huwe1 M1-S1000
Parent Plasmid
pCAGGS puro HA
Expressed
HA Huwe1 M1-S1000
DU Number
DU50837
Genbank
NM_031407.6 GI:531034782 with mutations -see notes
Tag
HA
Species
Human
Sequence of Insert
View Sequence
AGATCTATGAAAGTAGACAGGACTAAACTGAAGAAGACACCTACTGAGGCTCCTGCAGACTGCAGAGCCTTAATAGACAAACTCAAAGTTTGTAATGATGAGCAACTTCTCTTGGAACTGCAGCAGATCAAAACATGGAACATTGGAAAGTGCGAGTTATATCACTGGGTGGACCTGTTGGACCGCTTCGATGGAATACTGGCAGATGCTGGACAGACAGTGGAGAATATGTCATGGATGCTCGTATGTGATAGGCCAGAAAGAGAGCAACTGAAAATGCTTCTCTTGGCTGTGTTGAACTTCACAGCCTTGCTCATTGAGTACAGCTTTTCCCGGCATCTGTACAGTTCCATAGAGCATTTGACAACTTTATTGGCTTCCTCTGATATGCAAGTGGTGCTGGCAGTCCTCAATCTCCTATATGTATTTAGCAAAAGATCAAACTACATCACTCGTCTGGGATCTGACAAGAGGACCCCGCTGCTAACTCGGCTACAACATTTGGCAGAGAGCTGGGGTGGAAAGGAGAATGGCTTTGGACTTGCAGAATGTTGCAGAGACTTGCATATGATGAAATATCCACCCAGTGCAACTACACTACACTTTGAATTCTACGCAGATCCTGGGGCCGAGGTCAAAATTGAGAAAAGGACAACTAGTAACACACTACATTATATTCACATAGAGCAACTTGACAAGATTTCAGAAAGCCCTTCTGAAATCATGGAATCTCTTACCAAAATGTACAGCATTCCTAAGGATAAGCAGATGCTGTTATTTACACACATACGACTGGCCCATGGCTTTTCTAATCACAGGAAGCGATTGCAGGCAGTTCAGGCCAGACTGCATGCAATATCTATATTAGTGTATTCCAATGCCTTGCAGGAATCAGCAAACAGTATCTTGTATAATGGCTTGATAGAGGAGTTGGTAGATGTCCTTCAGATAACGGATAAGCAGCTTATGGAGATTAAAGCAGCTTCTTTACGAACATTAACATCAATTGTCCACTTGGAGAGAACTCCCAAACTCAGCAGTATTATTGACTGTACTGGAACTGCCTCCTACCATGGATTTTTGCCAGTGCTTGTAAGGAACTGTATCCAGGCCATGATTGATCCTTCCATGGATCCATACCCTCACCAGTTTGCCACTGCTCTCTTCTCTTTTTTATACCATCTGGCCAGCTACGATGCTGGTGGTGAAGCCTTGGTCTCCTGTGGAATGATGGAAGCCTTATTGAAGGTCATAAAGTTTCTTGGCGATGAACAGGACCAGATAACATTTGTCACCAGAGCCGTCAGAGTGGTTGACCTTATCACCAACCTGGATATGGCAGCTTTTCAATCCCATAGTGGACTTTCTATCTTCATTTATAGACTTGAGCATGAAGTAGATTTGTGCCGAAAAGAATGTCCGTTTGTGATCAAGCCAAAGATCCAGAGACCCAATACTACACAAGAAGGAGAGGAAATGGAAACTGATATGGATGGAGTCCAGTGTATTCCACAACGAGCAGTACTTCTGAAATCCATGTTGAATTTCCTCAAGAAGGCCATCCAAGACCCTACTTTCTCAGATGGCATACGACATGTGATGGATGGTTCTCTGCCTACCTCCCTGAAACACATCATCAGCAATGCAGAATACTATGGCCCATCACTCTTCCTCCTAGCTACTGAAGTGGTGACTGTGTTTGTATTTCAAGAACCATCACTGCTCTCCTCACTCCAGGACAATGGATTGACAGATGTCATGCTGCATGCACTGCTTATCAAAGATGTTCCTGCTACCCGTGAAGTCCTTGGCTCCCTCCCAAATGTATTCAGTGCACTCTGTTTGAATGCCCGAGGTCTTCAGTCTTTTGTTCAGTGTCAGCCTTTTGAACGCCTCTTCAAAGTTCTTCTGTCTCCAGATTACCTCCCAGCCATGCGGAGGAGGAGAAGTTCTGATCCCCTTGGGGATACTGCATCCAACCTGGGGAGTGCTGTCGATGAGCTCATGAGACATCAGCCCACCCTTAAAACAGATGCAACGACTGCCATCATCAAGTTACTTGAAGAAATCTGTAATCTTGGAAGGGACCCCAAATACATCTGTCAGAAGCCATCAATCCAGAAGGCAGATGGCACTGCCACTGCTCCTCCCCCAAGGTCTAATCATGCCGCAGAAGAAGCCTCTAGTGAGGATGAGGAGGAAGAGGAAGTACAGGCCATGCAGAGCTTTAATTCTACCCAGCAAAATGAAACTGAGCCTAATCAGCAGGTTGTTGGTACAGAGGAACGTATTCCTATTCCCCTCATGGATTACATCCTTAATGTGATGAAATTTGCGGAATCTATTCTGAGCAACAATACAACAGATGACCACTGCCAGGAATTTGTGAATCAGAAAGGACTGTTGCCTTTGGTTACCATTTTGGGTCTTCCCAATCTGCCCATTGACTTTCCCACATCTGCTGCCTGTCAGGCTGTTGCAGGTGTCTGCAAATCCATATTGACACTGTCACATGAACCCAAAGTCCTTCAAGAGGGTCTCCTTCAGTTGGACTCCATCCTCTCCTCCCTGGAGCCCTTACACCGCCCCATTGAATCCCCTGGGGGCTCAGTGTTGTTGCGAGAACTGGCTTGCGCAGGCAATGTTGCTGGTGCTACCCTCTCAGCCCAGGCCACACCTCTGCTGCATGCACTCACTGCTGCCCATGCCTACATCATGATGTTTGTTCATACTTGCAGAGTTGGACAGAGTGAAATTCGTTCCATCTCCGTAAACCAGTGGGGCTCTCAATTGGGTCTGAGTGTTTTGAGCAAGCTGAGCCAGTTATACTGTTCCCTGGTGTGGGAAAGCACTGTCCTCCTCTCTCTGTGTACCCCAAACAGCCTACCATCTGGGTGTGAATTTGGCCAGGCAGATATGCAGAAACTGGTTCCAAAGGATGAGAAGGCAGGTACGACCCAGGGTGGAAAAAGATCAGATGGGGAACAGGATGGAGCAGCTGGAAGTTAATCGCGA
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSMKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQ
LKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHM
MKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILV
YSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFS
FLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEG
EEMETDMDGVQCIPQRAVLLKSMLNFLKKAIQDPTFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHA
LLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEI
CNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFAESILSNNTTDD
HCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVAGATLSAQ
ATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGK
RSDGEQDGAAGS*
LKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHM
MKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILV
YSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFS
FLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEG
EEMETDMDGVQCIPQRAVLLKSMLNFLKKAIQDPTFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHA
LLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEI
CNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFAESILSNNTTDD
HCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVAGATLSAQ
ATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGK
RSDGEQDGAAGS*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
C506V
A523T
V777A
D882G