This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
MTMR15
Plasmid
pBabeD puro GFP mouse Fan1 R510H
Parent Plasmid
pBabeD puro GFP
Expressed
GFP mouse FAN1 R510H
DU Number
DU50731
Genbank
NM_177893.3 with a414g silent and c1125t silent
Tag
GFP
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGCCGTCACAAAGGAAATCACCTGATCAAAAAAGACCCCGTAGAAGCTTATCTACCAGCAAAACTGCAAAAAGCCAGTGTCATTCGATTACTTCGTATTTTAACAGTGCACCACCTGCTAAACTTGCATGTTCAACTTGTCATAAAATGGTGCCCAGGTATGACCTCATTCGGCACCTTGATGAGTCTTGTGCTAACAACGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGAGTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTGACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATGTGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATGCCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATTATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGCTAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGACTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATGGAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTCATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACAATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCCCTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTCAACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGCAAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTGCGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCTGGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTGACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGTGTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGAGACAGCTCAACTGGCACTGAGTCATCCGTACTACCTGCGGAGCTTCCTGGTGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGATGAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGGTCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAATGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTTGAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCTGAAAGCCCTGGCCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTAGACGCCTTTCACAAACTGGCCAAACAGCACTCAGTCTGCACGTGGGGCAAGACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGGCTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGAATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGCTTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCCGAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGGGACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGACATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTGCTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTGAGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTGGATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTCACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAAATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAACCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGGAAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCACCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGACCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTTGTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGTGGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGATTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGGGATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCAGGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGCAGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGCCTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGCCTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATCTTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTGGCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTGGAATTCTCAGAGCCACCATTGCAAGCTGGTGGAGGTGAAAGGCCCCAGTGATCGACTTTCATGTAAGCAGATGATCTGGCTGTACGAACTCCAAAAGCTGGGGGCTGATGTAGAAGTGTGCCATGTGGTTGCAGTTGGTGCTAAGAGCAAAGGTCTTGGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYD
LIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGS
LTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPAS
AEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCP
LEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKD
SGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQHSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLF
SLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEA
LPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVR
LRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFM
DGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFR
HCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGLG*
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYD
LIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGS
LTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPAS
AEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCP
LEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKD
SGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQHSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLF
SLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEA
LPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVR
LRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFM
DGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFR
HCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGLG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
Download datasheet