This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pGEX C3 HALO thrombin-N4BP1 Phe596-Asp896
Parent Plasmid
pGEX C3 HALO thrombin
Expressed
GST HALO N4BP1 Phe596-Asp896
DU Number
DU28006
Genbank
NM_153029.3
Tag
HALO
GST
Species
Human
Sequence of Insert
View Sequence
ggatcctttcgagatactctaaaaataccctacaagctggaattaaaaaa
tgaaccagggagaacggatttgaaacacattgttatagatgggagcaatg
ttgcaattacccatggtctgaaaaagttcttttcttgtcgtggaattgca
attgcagttgaatatttttggaagcttggcaacagaaacatcactgtatt
tgtccctcagtggagaacaaggcgtgatcctaatgtcacagaacagcact
tcttaacccagctccaggagctcggaatattatctttaactcctgcccgg
atggtctttggagaaagaattgcttctcatgatgacaggtttctactaca
cttagcggacaaaactggtggcataattgtgacaaatgataatttcagag
aatttgtgaatgagtcagtctcttggagagaaattattacaaaaaggctg
ctgcagtacacgttcgtgggggacatatttatggttcccgatgatcctct
gggaagaagtggacctcgattagaggagtttcttcagaaggaagtctgtc
ttagagatatgcagcccctactcagtgccctgccaaatgtgggcatgttt
gaccccagcttcagagtccctggcacccaggcagccagcaccagccacca
gcctccgacccggattcagggagccccttcaagccactggctccctcagc
agccccacttcccactgctgccagccctccccagtctccagcagaacctg
cccatgccagctcagagatcttctgcagaaaccaacgagctgagggaagc
ccttctgaagatcttccctgactcagagcaaagactgaaaatagaccaga
tcttggtggcccacccatacatgaaagatctaaatgcgctttctgccatg
gtgttggattgagcggccgc
tgaaccagggagaacggatttgaaacacattgttatagatgggagcaatg
ttgcaattacccatggtctgaaaaagttcttttcttgtcgtggaattgca
attgcagttgaatatttttggaagcttggcaacagaaacatcactgtatt
tgtccctcagtggagaacaaggcgtgatcctaatgtcacagaacagcact
tcttaacccagctccaggagctcggaatattatctttaactcctgcccgg
atggtctttggagaaagaattgcttctcatgatgacaggtttctactaca
cttagcggacaaaactggtggcataattgtgacaaatgataatttcagag
aatttgtgaatgagtcagtctcttggagagaaattattacaaaaaggctg
ctgcagtacacgttcgtgggggacatatttatggttcccgatgatcctct
gggaagaagtggacctcgattagaggagtttcttcagaaggaagtctgtc
ttagagatatgcagcccctactcagtgccctgccaaatgtgggcatgttt
gaccccagcttcagagtccctggcacccaggcagccagcaccagccacca
gcctccgacccggattcagggagccccttcaagccactggctccctcagc
agccccacttcccactgctgccagccctccccagtctccagcagaacctg
cccatgccagctcagagatcttctgcagaaaccaacgagctgagggaagc
ccttctgaagatcttccctgactcagagcaaagactgaaaatagaccaga
tcttggtggcccacccatacatgaaagatctaaatgcgctttctgccatg
gtgttggattgagcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSFRDTLKIPYKLELK
NEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITV
FVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLL
HLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDP
LGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSH
QPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELRE
ALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSFRDTLKIPYKLELK
NEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITV
FVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLL
HLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDP
LGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSH
QPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELRE
ALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Parental 24192
100042 R 1000 1001 1047 19007
Download datasheet