SHARPIN | Ubiquitylation Resource

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Synonyms

SHANK-associated RH domain interactor (SHARPIN), SIPL1

Plasmid

pCDFDuet-1-6His-HOIP UBA-RBR domain/ Sharpin E173-S346

Parent Plasmid

pSC-B

Expressed

Sharpin E173-S346

DU Number

DU24937

Genbank

AAH25244.1 NM_030974.4 → NP_112236.3

Species

Human

Sequence of Insert

View Sequence

CATATGGAAGAGCTGGCAGGGAGCCTGGCCCGGGCTATTGCAGGTGGAGACGAGAAGGGGGCAGCCCAAGTGGCAGCCGTCCTGGCCCAGCATCGTGTGGCCCTGAGTGTTCAGCTTCAGGAGGCCTGCTTCCCACCTGGCCCCATCAGGCTGCAGGTCACACTTGAAGACGCTGCCTCTGCCGCATCCGCCGCGTCCTCTGCACACGTTGCCCTGCAGGTCCACCCCCACTGCACTGTTGCAGCTCTCCAGGAGCAGGTGTTCTCAGAGCTCGGTTTCCCGCCAGCCGTGCAACGCTGGGTCATCGGACGGTGCCTGTGTGTGCCTGAGCGCAGCCTTGCCTCTTACGGGGTTCGGCAGGATGGGGACCCTGCTTTCCTCTACTTGCTGTCAGCTCCCCGAGAAGCCCCAGCCACAGGACCTAGCCCTCAGCACCCCCAGAAGATGGACGGGGAACTTGGACGCTTGTTTCCCCCATCATTGGGGCTACCCCCAGGCCCCCAGCCAGCTGCCTCCAGCCTGCCCAGTTAGCTCGAG

Amino Acid Sequence

View Sequence

MEELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRL
QVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWV
IGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDG
ELGRLFPPSLGLPPGPQPAASSLPS*

Vector Type

Bacterial

Antibiotic

Spectinomycin

Comments

silent change made at Pro303 (CCT > CCC) to knock out native Xho1 site at nt909.
Transcript Variant 1

100042 R 1000 1001 1047 19007

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