HOIP | Ubiquitylation Resource

Synonyms

ring finger protein 31 (RNF31) (HOIP)

Plasmid

pCDFDuet-1-6His-HOIP UBA-RBR domain/ Sharpin E173-S346

Parent Plasmid

pCDFDUET-1

Expressed

6His-HOIP UBA-RBR domain (aa474-1072)

DU Number

DU24937

Genbank

NM_017999.4

Tag

6His

Species

Human

Sequence of Insert

View Sequence

CCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGAAGTT
CTGTTCCAGGGGGGAGATCCTGAGAAGCAGCGCCAAGACAAGATGCGGGA
AGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAAGGGGAAGCCGCAGGTG
CCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTACTCGGGCACTGAGGTG
CCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACGTCCTGGAGATGGTGGC
TGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGTGCCTTTTCCTGTCAGG
AGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAACCTTGATGAAGCTGTG
GAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGCAGGAGCTCCAGTCTCT
AGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCATTGTTCCAGCACGGAG
GTGATGTGTCACGGGCCCTGACTGAGCTACAGCGCCAACGCCTAGAGCCC
TTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCCCTTCCTGGGA
TGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTTTTGGCAGTCTACGCAC
TCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTCACTGCTGCAGGAGACA
CCCAGGAACTATGAGTTGGGGGATGTGGTAGAAGCTGTGAGGCACAGCCA
GGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAGGAGTGTGCCGTGTGTG
GCTGGGCCCTGCCCCACAACCGGATGCAGGCCCTGACTTCCTGTGAGTGC
ACCATCTGTCCTGACTGCTTCCGCCAGCACTTCACCATCGCCTTGAAGGA
GAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCCCGACCTCA
CCGATGACACACAGTTGCTCAGCTACTTCTCTACCCTTGACATCCAGCTT
CGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGTTCCATAAGAAGCTGAC
CGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTGTGGTGTGCCCAGTGCT
CCTTTGGCTTCATATATGAGCGTGAGCAGCTGGAGGCAACTTGTCCCCAG
TGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCA
CCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGGAAACGCATGAACGACC
CAGAATACCAGGCCCAGGGCCTAGCAATGTATCTTCAGGAAAACGGCATT
GACTGCCCCAAATGCAAGTTCTCGTACGCCCTGGCCCGAGGAGGCTGCAT
GCACTTTCACTGTACCCAGTGCCGCCACCAGTTCTGCAGCGGCTGCTACA
ATGCCTTTTACGCCAAGAATAAATGTCCAGAGCCTAACTGCAGGGTGAAA
AAGTCCCTGCACGGCCACCACCCTCGAaACTGCCTCTTCTACCTGCGGGA
CTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAGGACAATAACGTCATGT
TTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGTCCCTGGAGGCGGCTGC
CGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGCTCAGGGACGAAGCTTG
TGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTGTGCCAGGCACACTACA
AAGAGTATCTTGTGAGCCTCATCAATGCCCACTCGCTGGACCCAGCCACC
TTGTATGAGGTGGAAGAGCTGGAGACGGCCACTGAGCGCTACCTGCACGT
ACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCTGCTTACCAGGCCCGCT
TGTTACAGAAGCTGACAGAAGAGGTACCCTTGGGACAGAGTATCCCCCGC
AGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGGDPEKQRQDKMREEGLQLVSMIREGEAAGA
CPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQE
ARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGG
DVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYAL
PSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCG
WALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLT
DDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCS
FGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDP
EYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYN
AFYAKNKCPEPNCRVKKSLHGHHPRNCLFYLRDWTALRLQKLLQDNNVMF
NTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYK
EYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARL
LQKLTEEVPLGQSIPRRRK*

Vector Type

Bacterial

Antibiotic

Spectinomycin

Comments

KO'd internal Xho1 site at nt2790 (>CTCGAA) relative to full-length sequence

100042 R 1000 1001 1047 19007