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Plasmid
pGEX_TEV-NEDD4L E576-D955 C922A
Parent Plasmid
pGEX TEV
Expressed
GST-NEDD4L E576-D955 C922A
DU Number
DU24934
Genbank
NP_056092
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GAATTCAAGCAGAAATATGACTACTTCAGGAAGAAATTAAAGAAACCTGC
TGATATCCCCAATAGGTTTGAAATGAAACTTCACAGAAATAACATATTTG
AAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGACCAGATGTCCTAAAA
GCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGGGG
TGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCT
ACTACGGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATC
AACCCTAATTCAGGCCTCTGTAATGAGGATCATTTGTCCTACTTCACTTT
TATTGGAAGAGTTGCTGGTCTGGCCGTATTTCATGGGAAGCTCTTAGATG
GTTTCTTCATTAGACCATTTTACAAGATGATGTTGGGAAAGCAGATAACC
CTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAATG
GATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACG
AAGAAAACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCA
GAAATAATGGTCACAAATGAAAACAAAAGGGAATATATCGACTTAGTCAT
CCAGTGGAGATTTGTGAACAGGGTCCAGAAGCAGATGAACGCCTTCTTGG
AGGGATTCACAGAACTACTTCCTATTGATTTGATTAAAATTTTTGATGAA
AATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAATGA
CTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCG
TCATTCAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGT
ATCCGGTTACTGCAGTTTGTCACAGGGACATCGCGAGTACCTATGAATGG
ATTTGCCGAACTTTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGC
AATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCACACAgcCTTTAATCGC
CTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTTCT
CATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAAGCGGCCG
C
TGATATCCCCAATAGGTTTGAAATGAAACTTCACAGAAATAACATATTTG
AAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGACCAGATGTCCTAAAA
GCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGGGG
TGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCT
ACTACGGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATC
AACCCTAATTCAGGCCTCTGTAATGAGGATCATTTGTCCTACTTCACTTT
TATTGGAAGAGTTGCTGGTCTGGCCGTATTTCATGGGAAGCTCTTAGATG
GTTTCTTCATTAGACCATTTTACAAGATGATGTTGGGAAAGCAGATAACC
CTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAATG
GATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACG
AAGAAAACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCA
GAAATAATGGTCACAAATGAAAACAAAAGGGAATATATCGACTTAGTCAT
CCAGTGGAGATTTGTGAACAGGGTCCAGAAGCAGATGAACGCCTTCTTGG
AGGGATTCACAGAACTACTTCCTATTGATTTGATTAAAATTTTTGATGAA
AATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAATGA
CTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCG
TCATTCAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGT
ATCCGGTTACTGCAGTTTGTCACAGGGACATCGCGAGTACCTATGAATGG
ATTTGCCGAACTTTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGC
AATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCACACAgcCTTTAATCGC
CTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTTCT
CATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAAGCGGCCG
C
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSPEFKQKYDYFRKKLKKPADIP
NRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAR
EWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGR
VAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILE
NDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWR
FVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQ
HSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAE
LYGSNGPQLFTIEQWGSPEKLPRAHTAFNRLDLPPYETFEDLREKLLMAV
ENAQGFEGVD*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSPEFKQKYDYFRKKLKKPADIP
NRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAR
EWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGR
VAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILE
NDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWR
FVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQ
HSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAE
LYGSNGPQLFTIEQWGSPEKLPRAHTAFNRLDLPPYETFEDLREKLLMAV
ENAQGFEGVD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
EcoR1 restriction site built into the second residue – silent change at Phe577 (TTT to TTC to incorporate EcoR1 site)
100042 R 1000 1001 1047 19007
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