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Synonyms
Cullin2
Plasmid
pET6HIS SUMO CUL2 1-277 (xenopus)
Parent Plasmid
pET15b 6His SUMO1
Expressed
6His SUMO Xenopus Cul2 1-277
DU Number
DU51229
Tag
SUMO
6His
Species
Xenopus laevis
Sequence of Insert
View Sequence
ATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTATGTTGAAAGAGCAA
CATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAGATATTTCTGGAAAA
CCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCAGAGGAGCTGATTAC
ATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGGAGTAGATATGAATG
AACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATGCTTCTCCGAGAAAT
TAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTA
AAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGAGTCAAACTGCTCAC
AATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTGATTCACGAATGCCA
GCAGAGAATGGTGGCAGATCACTTGCAGTTTTAACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCAACATGTATACTCTT
CTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAATCTTTCTCAGGAAA
ACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAGCATTTTATGAGTGC
ACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCAtgaGCGGCCGC
CATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAGATATTTCTGGAAAA
CCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCAGAGGAGCTGATTAC
ATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGGAGTAGATATGAATG
AACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATGCTTCTCCGAGAAAT
TAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTA
AAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGAGTCAAACTGCTCAC
AATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTGATTCACGAATGCCA
GCAGAGAATGGTGGCAGATCACTTGCAGTTTTAACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCAACATGTATACTCTT
CTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAATCTTTCTCAGGAAA
ACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAGCATTTTATGAGTGC
ACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCAtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSRGADY
MDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKKKFPL
KFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQF*
MDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKKKFPL
KFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQF*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please can you add 6His-SUMO (and no other amino acids) at the N-terminus of a fragment of Xenopus laevis Cul2, comprising amino acids 1-277. We already have a vector with the Xenopus Cul2 cDNA sequence. I want to make soluble protein and also generate antibodies.
100042 R 1000 1001 1047 19007
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