Cul2 (Xenopus) | Ubiquitylation Resource

Synonyms

Cullin2

Plasmid

pET6HIS SUMO Cul2 1-163 (xenopus)

Parent Plasmid

pET6HIS SUMO

Expressed

6His SUMO Xenopus_Cul2 1-163

DU Number

DU51228

Tag

SUMO

6His

Species

Xenopus laevis

Sequence of Insert

View Sequence

CCATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGAATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTA
TGTTGAAAGAGCAACATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAG
ATATTTCTGGAAAACCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCA
GAGGAGCTGATTACATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGG
AGTAGATATGAATGAACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATG
CTTTAACGAGAAATTAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGA
AAAAATTTCCCTTAAAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGA
GTCAAACTGCTCACAATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTG
ATTCACGAATGCCAGCAGAGAATGGTGGCAGATCACTTGCAGTTTCTACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCA
ACATGTATACTCTTCTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAA
TCTTTCTCAGGAAAACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAG
CATTTTATGAGTGCACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSR
GADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIML*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Please can you add 6His-SUMO (and no other amino acids) at the N-terminus of a fragment of Xenopus laevis Cul2, comprising amino acids 1-163. We already have a vector with the Xenopus Cul2 cDNA sequence. I want to make soluble protein and also generate antibodies.

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