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Synonyms
Cullin2
Plasmid
pET6HIS SUMO Cul2 1-383 V340R L344D
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Cul2 1-383 V340R L344D
DU Number
DU51239
Genbank
AAD23581.1
Tag
SUMO
6His
Species
Human
Sequence of Insert
View Sequence
ATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCAGACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAACAGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCACAGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGTCGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGAATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTACTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTCAGGAAAACATGCCAACACTATTTAGGGAGTCAGTTGATGAAGTGCATGGTAAATTTGTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTAAGTCTTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIVDNHTPKELGMEEEDVIEVYQE
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSK
GADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKK
KFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMAN
MYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFRESVDEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKS*
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSK
GADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKK
KFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMAN
MYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFRESVDEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please can you make 6His-SUMO tagged Human Cullin 2, residues 1-383, with the two mutations V340R and L344D. We already have a vector with a 6His-tagged version of this fragment.
100042 R 1000 1001 1047 19007
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