This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Synonyms
cullin2
Plasmid
pET15 6HIS SUMO Cul2 1-383 (Xenopus) V340R L344D
Parent Plasmid
pET 6HIS SUMO1
Expressed
6HIS SUMO Cul2 1-383 (Xenopus) V340R L344D
DU Number
DU51200
Tag
SUMO
6His
Species
Xenopus laevis
Sequence of Insert
View Sequence
ATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTATGTTGAAAGAGCAACATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAGATATTTCTGGAAAACCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCAGAGGAGCTGATTACATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGGAGTAGATATGAATGAACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATGCTTCTCCGAGAAATTAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGAGTCAAACTGCTCACAATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTGATTCACGAATGCCAGCAGAGAATGGTGGCAGATCACTTGCAGTTTCTACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCAACATGTATACTCTTCTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAATCTTTCTCAGGAAAACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAGCATTTTATGAGTGCACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCAtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSR
GADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKK
KFPLKFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQFLHAECHNIIRQERRNDMAN
MYTLLRAVSSGLPHMIQELQNHIHDEGLRAISNLSQENMPTQFRESVDEVHSKFVQLVNCVLNGDQHFMSALDKALTCVVNYREPKS*
QTGGMSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSR
GADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKK
KFPLKFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQFLHAECHNIIRQERRNDMAN
MYTLLRAVSSGLPHMIQELQNHIHDEGLRAISNLSQENMPTQFRESVDEVHSKFVQLVNCVLNGDQHFMSALDKALTCVVNYREPKS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please can you make 6His-SUMO tagged Xenopus laevis Cullin 2, residues 1-383, with the two mutations V340R and L344D. We already have a vector with a 6His-tagged version of this fragment.
100042 R 1000 1001 1047 19007
Download datasheet