USP47 | Ubiquitylation Resource

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Plasmid

pcDNA FRT TO GFP USP47 C177A (N500 restored)

Parent Plasmid

pcDNA5D FRT/TO GFP

Expressed

GFP-USP47 C177A

DU Number

DU24873

Genbank

NM_001282659.1 → NP_001269588.1 Gene ID 55031

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCCATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAAGAGAT
AGAAAATGCTGCTGAAGAACCTAGAGTCTTATGTATTATACAAGATACTA
CTAATTCAAAGACAGTGAATGAACGGATCACTTTAAATTTACCAGCATCT
ACTCCAGTCAGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACAT
AAATGGAACCTTTGACTTGGTGTGGGGAAATGGAATCAATACTGCTGATA
TGGCACCACTGGATCATACCAGTGACAAGTCACTTCTCGACGCTAATTTT
GAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACA
ACCTCAAATACTGCTGGAGGATTCCAGTGCTGGGGAAGACAGTGTTCATG
ACAGGTTTATAGGTCCGCTTCCAAGAGAAGGTTCTGGGGGTTCTACCAGT
GATTATGTCAGCCAAAGCTACTCCTACTCATCTATTTTGAATAAATCAGA
AACTGGATATGTGGGACTAGTAAACCAAGCAATGACTgcCTATTTGAATA
GCCTTTTGCAAACACTTTTTATGACTCCTGAATTTAGGAATGCATTATAT
AAGTGGGAATTTGAAGAATCTGAAGAAGATCCAGTGACAAGTATTCCATA
CCAACTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAA
TTGAAACCACAGATGTTACAAGGAGCTTTGGATGGGATAGTAGTGAGGCT
TGGCAGCAGCATGATGTACAAGAACTATGCAGAGTCATGTTTGATGCTTT
GGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTAT
ATCAAGGCAAGCTGAAGGACTACGTGAGATGTCTGGAATGTGGTTATGAG
GGCTGGCGAATCGACACATATCTTGATATTCCATTGGTCATCCGACCTTA
TGGGTCCAGCCAAGCATTTGCTAGTGTGGAAGAAGCATTGCATGCATTTA
TTCAGCCAGAGATTCTGGATGGCCCAAATCAGTATTTTTGTGAACGTTGT
AAGAAGAAGTGTGATGCACGGAAGGGCCTTCGGTTTTTGCATTTTCCTTA
TCTGCTGACCTTACAGCTGAAAAGATTCGATTTTGATTATACAACaATGC
ATAGGATTAAACTGAATGATCGAATGACATTTCCCGAGGAACTAGATATG
AGTACTTTTATTGATGTTGAAGATGAGAAATCTCCTCAGACTGAAAGTTG
CACTGACAGTGGAGCAGAAAATGAAGGTAGTTGTCACAGTGATCAGATGA
GCAACGATTTCTCCAATGATGATGGTGTTGATGAAGGAATCTGTCTTGAA
ACCAATAGTGGAACTGAAAAGATCTCAAAATCTGGACTTGAAAAGAATTC
CTTGATCTATGAACTTTTCTCTGTTATGGcTCATTCTGGGAGCGCTGCTG
GTGGTCATTATTATGCATGTATAAAGTCATTCAGTGATGAGCAGTGGTAC
AGCTTCAATGATCAACATGTCAGCAGGATAACACAAGAGGACATTAAGAA
AACACATGGTGGATCTTCAGGAAGCAGAGGATATTATTCTAGTGCTTTCG
CAAGTTCCACAAATGCATATATGCTGATCTATAGACTGAAGGATCCAGCC
AGAAATGCAAAATTTCTAGAAGTGtATGAATACCCAGAACATATTAAAAA
CTTGGTGCAGAAAGAGAGAGAGTTGGAAGAACAAGAAAAGAGACAACGAG
AAATTGAGCGCAATACATGCAAGATAAAATTATTCTGTTTGCATCCTACA
AAACAAGTAATGATGGAAAATAAATTGGAGGTTCATAAGGATAAGACATT
AAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAAGAGGTAA
TACCCCTGGATTGCTGTCGCCTTGTTAAATATGATGAGTTTCATGATTAT
CTAGAACGGTCATATGAAGGAGAAGAAGATACACCAATGGGGCTTCTACT
AGGTGGCGTCAAGTCAACATATATGTTTGATCTGCTGTTGGAGACGAGAA
AGCCTGATCAGGTTTTCCAATCTTATAAACCTGGAGAAGTGATGGTGAAA
GTTCATGTTGTTGATCTAAAGGCAGAATCTGTAGCTGCTCCTATAACTGT
TCGTGCTTACTTAAATCAGACAGTTACAGAATTCAAACAACTGATTTCAA
AGGCCATCCATTTACCTGCTGAAACAATGAGAATAGTGCTGGAACGCTGC
TACAATGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTGA
AGGATTTTTTAGAAGTAACAAGGTGTTTGTTGAAAGCTCCGAGACTTTGG
ATTACCAGATGGCCTTTGCAGACTCTCATTTATGGAAACTCCTGGATCGG
CATGCAAATACAATCAGATTATTTGTTTTGCTACCTGAACAATCCCCAGT
ATCTTATTCCAAAAGGACAGCATACCAGAAAGCTGGAGGCGATTCTGGTA
ATGTGGATGATGACTGTGAAAGAGTCAAAGGACCTGTAGGAAGCCTAAAG
TCTGTGGAAGCTATTCTAGAAGAAAGCACTGAAAAACTCAAAAGCTTGTC
ACTGCAGCAACAGCAGGATGGAGATAATGGGGACAGCAGCAAAAGTACTG
AGACAAGTGACTTTGAAAACATCGAATCACCTCTCAATGAGAGGGACTCT
TCAGCATCAGTGGATAATAGAGAACTTGAACAGCATATTCAGACTTCTGA
TCCAGAAAATTTTCAGTCTGAAGAACGATCAGACTCAGATGTGAATAATG
ACAGGAGTACAAGTTCAGTGGACAGTGATATTCTTAGCTCCAGTCATAGC
AGTGATACTTTGTGCAATGCAGACAATGCTCAGATCCCTTTGGCTAATGG
ACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACGAAAGCAAATGAAG
GGAAAAAAGAAACATGGGATACAGCAGAAGAAGACTCTGGAACTGATAGT
GAATATGATGAGAGTGGCAAGAGTAGGGGAGAAATGCAGTACATGTATTT
CAAAGCTGAACCTTATGCTGCAGATGAAGGTTCTGGGGAAGGACATAAAT
GGTTGATGGTGCATGTTGATAAAAGAATTACTCTGGCAGCTTTCAAACAA
CATTTAGAGCCCTTTGTTGGAGTTTTGTCCTCTCACTTCAAGGTCTTTCG
AGTGTATGCCAGCAATCAAGAGTTTGAGAGCGTCCGGCTGAATGAGACAC
TTTCATCATTTTCTGATGACAATAAGATTACAATTAGACTGGGGAGAGCA
CTTAAAAAAGGAGAATACAGAGTTAAAGTATACCAGCTTTTGGTCAATGA
ACAAGAGCCATGCAAGTTTCTGCTAGATGCTGTGTTTGCTAAAGGAATGA
CTGTACGGCAATCAAAAGAGGAATTAATTCCTCAGCTCAGGGAGCAATGT
GGTTTAGAGCTCAGTATTGACAGGTTTCGTCTAAGGAAAAAAACATGGAA
GAATCCTGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTA
ATATTTCCAGCAACTGGGAGGTTTTCCTTGAAGTTCTTGATGGGGTAGAG
AAGATGAAGTCCATGTCACAGCTTGCAGTTTTGTCAAGACGGTGGAAGCC
TTCAGAGATGAAGTTGGATCCCTTCCAGGAGGTTGTATTGGAAAGCAGTA
GTGTGGACGAATTGCGAGAGAAGCTTAGTGAAATCAGTGGGATTCCTTTG
GATGATATTGAATTTGCTAAGGGTAGAGGAACATTTCCCTGTGATATTTC
TGTCCTTGATATTCATCAaGATTTAGACTGGAATCCTAAAGTTTCTACgC
TGAATGTCTGGCCTCTTTATATCTGTGATGATGGTGCGGTCATATTTTAT
AGGGATAAAACAGAAGAATTAATGGAAcTGACAGATGAGCAAAGAAATGA
ACTGATGAAAAAAGAAAGCAGTCGACTCCAGAAGACTGGACATCGTGTAA
CATACTCACCTCGTAAAGAGAAAGCACTAAAAATATATCTGGATGGAGCA
CCAAATAAAGATCTGACTCAAGACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LEKLAAAMVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLN
LPASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLL
DANFEPGKKNFLHLTDKDGEQPQILLEDSSAGEDSVHDRFIGPLPREGSG
GSTSDYVSQSYSYSSILNKSETGYVGLVNQAMTAYLNSLLQTLFMTPEFR
NALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWD
SSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLE
CGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYF
CERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPE
ELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEG
ICLETNSGTEKISKSGLEKNSLIYELFSVMAHSGSAAGGHYYACIKSFSD
EQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRL
KDPARNAKFLEVYEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFC
LHPTKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEEVIPLDCCRLVKYDE
FHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQVFQSYKPGE
VMVKVHVVDLKAESVAAPITVRAYLNQTVTEFKQLISKAIHLPAETMRIV
LERCYNDLRLLSVSSKTLKAEGFFRSNKVFVESSETLDYQMAFADSHLWK
LLDRHANTIRLFVLLPEQSPVSYSKRTAYQKAGGDSGNVDDDCERVKGPV
GSLKSVEAILEESTEKLKSLSLQQQQDGDNGDSSKSTETSDFENIESPLN
ERDSSASVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILS
SSHSSDTLCNADNAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDS
GTDSEYDESGKSRGEMQYMYFKAEPYAADEGSGEGHKWLMVHVDKRITLA
AFKQHLEPFVGVLSSHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIR
LGRALKKGEYRVKVYQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQL
REQCGLELSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVL
DGVEKMKSMSQLAVLSRRWKPSEMKLDPFQEVVLESSSVDELREKLSEIS
GIPLDDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGA
VIFYRDKTEELMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIY
LDGAPNKDLTQD*

Vector Type

Mammalian

Antibiotic

Amp

Comments

isoform 4 (also known as isoform a). silent changes at T379, Q1270, T1280 and L1307 and non-silent mutations at V474>A, D556>Y
NCBI updated changes G814>V, Y1029>H are also confirmed in this construct.

100042 R 1000 1001 1047 19007

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