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Synonyms
CG13745; Dmel\CG13745; fanci
Plasmid
pBacPAK? 6His-GST 3C (no linker) Drosophila FancI M1- E1300
Expressed
6His-GST 3C (no linker) Drosophila FancI M1- E1300
DU Number
DU50422
Genbank
NM_136585.3 GI:665399661 with changes - see notes
Tag
GST
6His
Species
Drosophila Melanogaster
Amino Acid Sequence
View Sequence
MGHHHHHHGSTMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKER
AEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQI
DKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPESKDALAKRF
ECVRSFMDGLSSTELSYKQTFDLITRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRDTTVRSIATM
QWPASILTPIADMFKTMNLSSGEIVTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLDSIDLFSDKE
LREAEETILHHLNYCTMYKLTEKHLAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVWYRDTLQRKQ
VDLQQVLTVLIDQNKDGKDVITPGLVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADTYTVSECMKP
IQSVLEHMQWLSGEQSMRMMNFILPLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGYSIMTQNTLG
SRSNPQRNFDMLTLEIIGMLRNCLQQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSSDDQHMEIQL
KDNLGKLIQFVANCLAVFDRAPSGYDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQILPLFNQHCK
LLESLKALSDASKKAHKQLKQTAKNNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLSVTASRVESI
RLEPDYKQLAYSKRTFKQLTDITKVIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYTIQDVLDDFM
TEYGTDDSICKNEFVSKLPVYLLESLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHVLGVQNEDAP
SESVELTLKSISTVTIESCLQHLYAAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPLGSLMDGLMK
LLIQHYTCLKSLTKHYISSYTSDTSIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKVIMVIEDFNK
HIILLSKKTKSKNRLTDYLHLGTMRDFDIKSTDLRAAIE*
AEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQI
DKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPESKDALAKRF
ECVRSFMDGLSSTELSYKQTFDLITRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRDTTVRSIATM
QWPASILTPIADMFKTMNLSSGEIVTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLDSIDLFSDKE
LREAEETILHHLNYCTMYKLTEKHLAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVWYRDTLQRKQ
VDLQQVLTVLIDQNKDGKDVITPGLVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADTYTVSECMKP
IQSVLEHMQWLSGEQSMRMMNFILPLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGYSIMTQNTLG
SRSNPQRNFDMLTLEIIGMLRNCLQQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSSDDQHMEIQL
KDNLGKLIQFVANCLAVFDRAPSGYDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQILPLFNQHCK
LLESLKALSDASKKAHKQLKQTAKNNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLSVTASRVESI
RLEPDYKQLAYSKRTFKQLTDITKVIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYTIQDVLDDFM
TEYGTDDSICKNEFVSKLPVYLLESLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHVLGVQNEDAP
SESVELTLKSISTVTIESCLQHLYAAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPLGSLMDGLMK
LLIQHYTCLKSLTKHYISSYTSDTSIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKVIMVIEDFNK
HIILLSKKTKSKNRLTDYLHLGTMRDFDIKSTDLRAAIE*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Various silent changes, plus residue differences:
A8G
P224T
K947M.
Recommend growth at 30 degrees.