HOIP

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Synonyms
ring finger protein 31 (RNF31) (HOIP)
Plasmid
pcDNA5-FRT/TO-GFP HOIP (delta Xho1)
Parent Plasmid
pcDNA5 FRT/TO GFP
Expressed
GFP-HOIP
DU Number
DU24853
Genbank
NM_017999.4
Tag
GFP
Species
Human
Sequence of Insert
GCGGCCGCGATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCG
CGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCC
TGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGC
TACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGA
GCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGA
AATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGT
GTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGG
GGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATG
GGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCT
ACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATT
GCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACA
AGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGA
GAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCC
TGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCAT
CCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACAC
CCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGG
TACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGT
CGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCT
GCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGA
GCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGG
GGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCT
GATCTTGCACGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGA
GGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGC
CTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCC
CTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTA
CTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTA
TGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCC
TATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACG
CCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCC
AAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAA
GGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTA
CTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACG
TCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGT
GCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAA
CCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGC
AGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCA
TTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTACAGCG
CCAACGCCTAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGC
CCACCCCTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTT
TTGGCAGTCTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTC
ACTGCTGCAGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAG
CTGTGAGGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAG
GAGTGTGCCGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCT
GACTTCCTGTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCA
CCATCGCCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGT
GGCCGCCCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTAC
CCTTGACATCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGT
TCCATAAGAAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTG
TGGTGTGCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGA
GGCAACTTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCC
AGTGGGAGGAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGG
AAACGCATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCT
TCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGG
CCCGAGGAGGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTC
TGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCC
TAACTGCAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAaACTGCC
TCTTCTACCTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAG
GACAATAACGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGT
CCCTGGAGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGC
TCAGGGACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTG
TGCCAGGCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTC
GCTGGACCCAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTG
AGCGCTACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCT
GCTTACCAGGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGG
ACAGAGTATCCCCCGCAGGCGGAAGTAGGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAA
RYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWR
GVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQV
ATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLL
REIAPGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHG
HPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPN
PASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLE
PDLARGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQ
PLQQGDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPR
PYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIR
EGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGL
GAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQ
ALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRR
LLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLA
QECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPA
CGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKF
LWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQN
WKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQ
FCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRNCLFYLRDWTALRLQKLL
QDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAG
LCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDP
PAYQARLLQKLTEEVPLGQSIPRRRK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
KO'd internal Xho1 site at nt2790 (>CTCGAA)
*although has another Xho1 site at nt1271*
100042 R 1000 1001 1047 19007
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