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Synonyms
NY-REN-60; USP10
Plasmid
pcDNA5 FRT/TO-GFP USP32
Parent Plasmid
pcDNA FRT TO GFP
Expressed
GFP USP32
DU Number
DU24841
Genbank
NM_032582.3 GI:44889413
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
CTCGAGATGGGTGCCAAGGAGTCACGGATCGGATTCCTCAGCTACGAGGAGGCGCTGAGGAGAGTTACAGATGTAGAGCTAAAACGACTGAAGGATGCTTTCAAGAGGACCTGTGGACTCTCATATTACATGGGCCAGCACTGCTTCATCCGGGAAGTGCTTGGGGATGGAGTGCCTCCAAAGGTTGCTGAGGTGATTTACTGTTCTTTTGGTGGAACATCCAAAGGGCTGCACTTCAATAATTTAATAGTTGGACTTGTCCTCCTTACAAGAGGCAAAGATGAAGAGAAAGCAAAATACATTTTTAGTCTTTTTTCAAGTGAATCTGGGAACTATGTTATACGGGAAGAAATGGAAAGAATGCTCCACGTGGTGGATGGTAAAGTCCCAGATACACTCAGGAAGTGTTTCTCAGAGGGTGAAAAGGTAAACTATGAAAAGTTTAGAAATTGGCTTTTTCTAAACAAAGATGCTTTTACTTTCTCTCGATGGCTTCTATCTGGAGGTGTGTATGTTACCCTCACTGATGATAGTGATACTCCTACTTTCTACCAAACTCTGGCTGGAGTCACACATTTGGAGGAATCAGACATCATTGATCTTGAGAAACGCTATTGGTTATTGAAGGCTCAATCCCGGACTGGACGATTTGATTTAGAGACATTTGGCCCATTGGTTTCACCACCTATTCGTCCATCTCTAAGTGAAGGTTTGTTTAATGCTTTTGATGAAAATCGTGACAATCACATAGATTTTAAGGAGATATCCTGTGGGTTATCAGCCTGTTGCAGGGGACCCCTGGCTGAAAGACAAAAATTTTGCTTCAAGGTATTTGATGTTGACCGTGATGGAGTTCTCTCCAGGGTTGAACTGAGAGACATGGTGGTTGCACTTTTAGAAGTCTGGAAGGACAACCGCACTGATGATATTCCTGAATTACATATGGATCTCTCTGATATTGTAGAAGGCATACTGAATGCACATGACACCACAAAGATGGGTCATCTTACTCTGGAAGACTATCAGATCTGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAACCTCCTTTTCCAGGTGTGTCACATAGTTCTGGGGTTAAGACCAGCTACTCCGGAAGAAGAAGGACAAATTATTAGAGGATGGTTAGAACGAGAGAGCAGGTATGGTCTGCAAGCAGGACACAACTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATACGATGCCAACCCTGTGGTAATTGAGCCATCATCTGTTTTGAATGGAGGAAAATACTCATTTGGAACTGCAGCCCATCCTATGGAGCAGGTCGAAGATAGAATTGGAAGCAGCCTCAGTTACGTGAATACTACAGAAGAGAAATTTTCAGACAACATTTCTACTGCATCTGAAGCCTCAGAAACTGCTGGCAGCGGCTTTCTGTATTCTGCCACACCAGGGGCAGATGTTTGCTTTGCTCGACAACATAACACTTCTGACAATAACAACCAGTGTTTGCTGGGAGCCAATGGGAATATTTTGTTGCACCTTAACCCTCAGAAACCAGGGGCTATTGATAATCAGCCATTAGTAACTCAAGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGACGATTAAAACGAACTCCACAGCTGATTCATGGAAGAGACTATGAAATGGTCCCAGAACCTGTGTGGAGAGCACTTTATCACTGGTATGGAGCAAACCTGGCCTTACCTAGACCAGTTATCAAGAACAGCAAGACAGACATCCCAGAGCTGGAATTATTTCCCCGCTATCTTCTCTTCCTGAGACAGCAGCCTGCCACTCGGACACAGCAGTCTAACATCTGGGTGAATATGGGAAATGTACCTTCTCCGAATGCACCTTTAAAGCGGGTATTAGCCTATACAGGCTGTTTTAGTCGAATGCAGACCATCAAGGAAATTCACGAATATCTATCTCAAAGGCTGCGCATTAAAGAGGAAGATATGCGCCTGTGGCTATACAACAGTGAGAACTACCTTACTCTTCTGGATGATGAGGATCATAAATTGGAATATTTGAAAATCCAGGATGAACAACACCTGGTAATTGAAGTTCGCAACAAAGATATGAGTTGGCCTGAGGAGATGTCTTTTATAGCAAATAGTAGTAAAATAGATAGACACAAGGTTCCCACAGAAAAGGGAGCCACAGGTCTAAGCAATCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTTAGTAACACACAGCCACTGACACAGTATTTTATCTCAGGGAGACATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAGGGGCATATGGCTAAATGCTATGGTGATTTAGTGCAGGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCATTAAAGCTTCGGTGGACCATAGCAAAATATGCTCCCAGGTTTAATGGGTTTCAGCAACAGGACTCCCAAGAACTTCTGGCTTTTCTCTTGGATGGTCTTCATGAAGATCTTAATCGAGTCCATGAAAAGCCATATGTGGAACTGAAGGACAGTGATGGGCGACCAGACTGGGAAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGAAGAAATAGATCAATTGTTGTGGATTTGTTCCATGGGCAGCTAAGATCTCAAGTAAAATGCAAGACATGTGGGCATATAAGTGTCCGATTTGACCCTTTCAATTTTTTGTCTTTGCCACTACCAATGGACAGTTATATGCACTTAGAAATAACAGTGATTAAGTTAGATGGTACTACCCCTGTACGGTATGGACTAAGACTGAATATGGATGAAAAGTACACAGGTTTAAAAAAACAGCTGAGTGATCTCTGTGGACTTAATTCAGAACAAATCCTTCTAGCAGAAGTACATGGTTCCAACATAAAGAACTTTCCTCAGGACAACCAAAAAGTACGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTGTCCCTGTGTCTCCAATTTCAGCTTCTAGTCCAACACAGACAGATTTCTCCTCTTCGCCATCTACAAATGAAATGTTCACCCTAACTACCAATGGGGACCTACCCCGACCAATATTCATCCCCAATGGAATGCCAAACACTGTTGTGCCATGTGGAACTGAGAAGAACTTCACAAATGGAATGGTTAATGGTCACATGCCATCTCTTCCTGACAGCCCCTTTACAGGTTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCGCCCCAGCCTCTTTGGAATGCCATTGATTGTTCCATGTACTGTGCATACCCGGAAGAAAGACCTATATGATGCGGTTTGGATTCAAGTATCCCGGTTAGCGAGCCCACTCCCACCTCAGGAAGCTAGTAATCATGCCCAGGATTGTGACGACAGTATGGGCTATCAATATCCATTCACTCTACGAGTTGTGCAGAAAGATGGGAACTCCTGTGCTTGGTGCCCATGGTATAGATTTTGCAGAGGCTGTAAAATTGATTGTGGGGAAGACAGAGCTTTCATTGGAAATGCCTATATCGCTGTGGATTGGGATCCCACAGCCCTTCACCTTCGCTATCAAACATCCCAGGAAAGGGTTGTAGATGAGCATGAGAGTGTGGAGCAGAGTCGGCGAGCGCAAGCCGAGCCCATCAACCTGGACAGCTGTCTCCGTGCTTTCACCAGTGAGGAAGAGCTAGGGGAAAATGAGATGTACTACTGTTCCAAGTGTAAGACCCACTGCTTAGCAACAAAGAAGCTGGATCTCTGGAGGCTTCCACCCATCCTGATTATTCACCTTAAGCGATTTCAATTTGTAAATGGTCGGTGGATAAAATCACAGAAAATTGTCAAATTTCCTCGGGAAAGTTTTGATCCAAGTGCTTTTTTGGTACCAAGAGACCCGGCTCTCTGCCAGCATAAACCACTCACACCCCAGGGGGATGAGCTCTCTGAGCCCAGGATTCTGGCAAGGGAGGTGAAGAAAGTGGATGCGCAGAGTTCGGCTGGGGAAGAGGACGTGCTCCTGAGCAAAAGCCCATCCTCACTCAGCGCTAACATCATCAGCAGCCCGAAAGGTTCTCCTTCTTCATCAAGAAAAAGTGGAACCAGCTGTCCCTCCAGCAAAAACAGCAGCCCTAATAGCAGCCCACGGACTTTGGGGAGGAGCAAAGGGAGGCTCCGGCTGCCCCAGATTGGCAGCAAAAATAAACTGTCAAGTAGTAAAGAGAACTTGGATGCCAGCAAAGAAAATGGGGCTGGGCAGATATGTGAGCTGGCTGACGCCTTGAGTCGAGGGCATGTGCTGGGGGGCAGCCAACCAGAGTTGGTCACTCCTCAGGACCATGAGGTAGCTTTGGCCAATGGATTCCTTTATGAGCATGAAGCATGTGGCAATGGCTACAGCAATGGTCAGCTTGGAAACCACAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGATACTCGTATTAAGCCTATTTATAATCTATATGCAATTTCGTGCCATTCAGGAATTCTGGGTGGGGGCCATTACGTCACTTATGCCAAAAACCCAAACTGCAAGTGGTACTGTTACAATGACAGCAGCTGTAAGGAACTTCACCCGGATGAAATTGACACCGACTCTGCCTACATTCTTTTCTATGAGCAGCAGGGGATAGACTATGCACAATTTCTGCCAAAGACTGATGGCAAAAAGATGGCAGACACAAGCAGTATGGATGAAGACTTTGAGTCTGATTACAAAAAGTACTGTGTGTTACAGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LEMGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFI
REVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKY
IFSLFSSESGNYVIREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRN
WLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIID
LEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHI
DFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLE
VWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVL
ANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFI
ISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKYSFGTAAHPMEQVEDRIG
SSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTS
DNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLK
RTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPELELF
PRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTI
KEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHL
VIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSS
IQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGT
QKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP
YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGH
ISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLNMDEKYTG
LKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIP
VPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVP
CGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRP
SLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDS
MGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNAYIAVD
WDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEEL
GENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKI
VKFPRESFDPSAFLVPRDPALCQHKPLTPQGDELSEPRILAREVKKVDAQ
SSAGEEDVLLSKSPSSLSANIISSPKGSPSSSRKSGTSCPSSKNSSPNSS
PRTLGRSKGRLRLPQIGSKNKLSSSKENLDASKENGAGQICELADALSRG
HVLGGSQPELVTPQDHEVALANGFLYEHEACGNGYSNGQLGNHSEEDSTD
DQREDTRIKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKE
LHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSSMDEDFESDYK
KYCVLQ*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LEMGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFI
REVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKY
IFSLFSSESGNYVIREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRN
WLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIID
LEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHI
DFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLE
VWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVL
ANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFI
ISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKYSFGTAAHPMEQVEDRIG
SSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTS
DNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLK
RTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPELELF
PRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTI
KEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHL
VIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSS
IQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGT
QKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKP
YVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGH
ISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLNMDEKYTG
LKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIP
VPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVP
CGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRP
SLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDS
MGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNAYIAVD
WDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEEL
GENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKI
VKFPRESFDPSAFLVPRDPALCQHKPLTPQGDELSEPRILAREVKKVDAQ
SSAGEEDVLLSKSPSSLSANIISSPKGSPSSSRKSGTSCPSSKNSSPNSS
PRTLGRSKGRLRLPQIGSKNKLSSSKENLDASKENGAGQICELADALSRG
HVLGGSQPELVTPQDHEVALANGFLYEHEACGNGYSNGQLGNHSEEDSTD
DQREDTRIKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKE
LHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSSMDEDFESDYK
KYCVLQ*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 41455
100042 R 1000 1001 1047 19007
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