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Plasmid
pGEX6P1 Halo chicken FancI
Parent Plasmid
pGEX6P1 Halo
Expressed
GST-3C-HALO-FANCI
DU Number
DU50351
Genbank
NM_001114851.1 GI:168803993 with killed internal BamHI
Tag
GST
HALO
Species
Chicken
Sequence of Insert
View Sequence
GGATCCATGGCGCAGCGCATCCTGCAGCTGGCGGCCGAGGGGAGCCCCGAGCGGCTCCAGGAGGCTCTTCAGGGCCTGACGGAGGGCGAGCTGGGTGACATGGTGACGAGGCAGGCCCTGAGGGGCAGGGAGACGGCAGCGCTGCTTAAGGGGATCTTCAAAGGCTCCCCATGTTCCCAGCAAAGCGGTGTTCTCAGGAGGCTGCAGGTTTACAAGCACTGTGTCTCACTGGTGGAATCAGGAGATCTGCATGTGGGCAAAGTGTCCGAAATCATAGGGCTGCTGATGCTGGAGGCTCGCCAGCTGCCAGGCCATGCGTTGGCTGAACTTGCCACCTTGTTTGTTGAAGTTATTAAGAGAGGCAGCCTGTCTAATGGGAAATCCTTGGAGTTGTTTTCCACCGTTCTTACTGCGTTGTCCAACTCTAAGGAGAGCCTGGCTTATGGGAAAGGTGAACTGAACGGGGAAGAATTTAAGAAACAGCTGATAAATACCCTCTGCTCCAGCAAGTGGGACCCTCAGTGTGTAATACATCTTGCCAACATGTTCAGGGATATTCCACTATCAGGAGAGGAGCTGCAGTTTGTGGTGGAAAAGGTCCTTAGGATGTTCTCCAAATTAGACCTGCAGGAAATACCCCCCCTGGTCTATCAGCTGCTGCTGCTTTCTGCAAAGGGCAGTAAGAAAACTGTTTTAGAAGGAATCATCAGTTTTTTCAATCAGTTGGACAAGAGACAAAAGGAAGAGCAGAGGGTTCCTCAATCAGCAGACCTTGAGGTAGCCACAGTGCCCCTAGACCAGCTCCGCCACGTGGAAGGTACCGTTATCCTCCACATTGTTTCTGCTATCAACCTGGATCAGGACATTGGTGAGGAGCTCATCAAACATCTGAAGACTGAGCAACAGAAGGACCCTGGTAAAGCCCTGTGTCCCTTCAGTGTGTCCCTTTTGCTTTCGACTGCAGTGAAACACAGACTGCAAGAGCAGATATTTGATTTCTTGAAAACATCAATCACGAGAAGCTGCAAGGACCTGCAGATCCTACAGGCCTCCAAGTTTCTGCAGGATTTGTGTCCTCAGCAGTATGATGTAACAGCTGTAATTCTGGAAGTGGTGAAAAATAGTGCTTTTGGCTGGGACCATGTTACCCAGGGACTGGTAGATCTTGGCTTCAGCCTAATGGAATCGTATGAACCAAAAAAGTCCTTTGGAGGGAAAGCTGCTGAGACCAATTTGGGCCTTTCAAAAATGCCAGCACAGCAGGCTTGCAAACTGGGAGCGAGTATCCTGCTGGAAACCTTTAAGGTCCATGAGCCCATCAGAAGTGATATTCTTGAGCAAGTCCTGAACAGGGTCCTCACAAAAGCAGCATCTCCTGTCAGCCACTTCATAGACTTGCTGTCCAATATTGTTGTGTCTGCTCCTCTTGTGCTTCAGAACTCATCCTCCAGAGTCACAGAGACCTTTGACAATTTGTCCTTTCTGCCCATCGACACAGTGCAAGGGCTCCTCCGGGCAGTACAGCCCCTGCTCAAAGTCAGCATGTCGGTGAGGGACTCCCTGATACTTGTTCTCCAGAAAGCTATCTTTTCCAGGCAGCTCGATGCTCGTAAAGCTGCAGTTGCTGGCTTTTTGCTTCTGTTAAGAAATTTTAAGATTCTGGGCAGCTTGACTTCTTCCCAGTGCAGCCAGGCCATTGGTGCCACTCAGGTCCAGGCAGATGTTCATGCCTGCTATAATTCTGCAGCTAATGAGGCCTTCTGCCTTGAAATCCTGGGCAGCCTGAGGCGATGTCTGAGCCAGCAAGCAGATGTCCGACTCATGTTATATGAGGGTTTTTATGATGTCCTACGCAGGAACTCCCAGCTGGCCAGCTCTATAATGGAAACACTTTTGTCCCAGATAAAGCAATACTACTTGCCCCAGCAAGATCTCCTGCCTCCACTGAAACTTGAGGGATGTATAATGGCTCAAGGAGATCAAATCTTTTTGCAAGAACCACTGGCCCATCTGCTCTGCTGCATCCAGCACTGTCTAGCCTGGTATAAGAGCACTGTGCATCTATGCAAAGGAGCTGAAGATGAGGAGGAGGAGGAGGATGTGGGATTTGAGCAAAACTTTGAAGAGATGTTAGAATCTGTCACACGACGGATGATCAAGAGTGAGCTGGAAGACTTTGAACTGGATAAATCAGCAGATTTTTCTCCATCTTCTGGTGTTGGTGTAAAGAATAACATCTATGCCATCCAGGTGATGGGAATTTGCGAGGTCTTGATTGAGTACAATTTCAAAATAGGGAATTTCAGTAAGAACAAATTTGAAGATGTCCTAGGCCTGTTTACATGTTACAACAAGCTCTCAGAAATCTTGAAGGAGAAAGCTGGAAAGAATAAATCTACGTTGGGCAACAGAATTGCACGGAGCTTCCTCTCTATGGGTTTTGTGTCTACGCTGCTCACAGCTCTGTTCAGGGACAATGCTCAAAGCCACGAGGAGAGCCTGGCAGTGCTGCGCTCCAGCACGGAGTTCATGCGCTACGCTGTCAGTGTGGCACTACAGAAGGTGCAGCAGCTGGAGGAGATGGGACAAACCGACGGCCCGGATGGACAAAACCCTGAGAAGATGTTTCAGAACCTCTGCAAAATCACACGGGTTCTGCTTTGGAGATACACTTCAATTCCCACTGCTGTTGAAGAGTCAGGGAAGAAGAAGGGCAAAAGCATTTCCCTGCTGTGCTTGGAAGGTTTGCTGCGGATCTTCAACACCATGCAGCAGCTGTACGCTGCTAGGATTCCCCAGTTTCTACAGGCCCTGGATATTACTGATGGTGACGCAGAAGAAGCGGATATTAATGTCACAGAGAAAGCTGCCTTCCAGATCCGACAGTTTCAGAGGTCTCTGGTGAACCAGCTCAGTAGTGCTGAAGATGACTTCAACTCCAAGGAAACACAGTTACTCATCACAATCCTCTCCACTTTGTCCAAACTCCTGGACCCAGGCTCTCAGCAGTTCCTTCAGTTCCTTACTTGGACAGTCAAAATTTGCAAAGAAAATGCTTTAGAGGATCTCTCTTGCTGTAAGGGTTTGCTGACTTTGCTTTTTAGTCTCCATGTTCTGTACAAGAGTCCTGTCAGTCTGCTGCGTGAACTTGCTCAAGATATCCATGCTTGCCTGGGAGACATAGATCAGGATGTAGAAATAGAGAGTCGATCCCATTTTGCCATAGTGAATGTGAAGACTGCAGCCCCTACTGTCTGCCTGCTGGTTCTGGGGCAGGCAGATAAGGTCCTTGAAGAGGTGGATTGGCTTATCAAGAGGCTTACCATCCTGGGGTCAGACACATCAGAAGACTCGACTCAGGCATCAAACCAAACCCAGGCTCTGGAGAAAGGTGTGATTCTGCAGTTGGGAACTCTGCTGACAGTTTTTCATGAGCTGGTGCAGACAGCACTTCCTGCAGGGAGCTGTGTAGACTCGCTGCTGAGAAGCCTCAGCAAGACGTATGCAATCCTCACCTCCCTCATCAAGCATTATATCCAAGCTTGCCGCAGCACCTCAAATACGGTTCCAGGGAGGCTTGAAAAGCTGGTGAAGCTCTCGGGTTCCCATTTGACCCCACAGTGTTACTCATTCATTACTTATGTACAGAACATCCATAGTGAGAGCTTAAGCTTTGCAGAAGAGAAGAAAAAGAAGAAGAAAGAAGACGAAACTGCTGTAGTCTCCACAGTCATGGCTAAGGTGCTTCGGGACACCAAGCCAATTCCAAACCTGATTTTTGCTATAGAGCAATACGAGAAGTTCCTTATCCATCTTTCGAAGAAATCAAAGGTGAACTTGATGCAATATATGAAACTAAGCACCTCCCGGGACTTCCGCATCAACGCATCCATGCTAGACAGTGTGCTGCAAGAGCAGAACACAGAGGATGCTGAAAATGAACCAGACAACAACCAGTCCGGCACAGCAGAGCAGCCAGATGAAAACCAGGAACCCCAAAAGAAAAGAAGGCGAAAAAAATAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGGSMAQRILQLAAEGSPERLQEALQGLTEGELGDMVTRQALRGRETAALLKGIFKGSPCSQQSGVLRRLQVYK
HCVSLVESGDLHVGKVSEIIGLLMLEARQLPGHALAELATLFVEVIKRGSLSNGKSLELFSTVLTALSNSKESLAYGKGELNGEEFKKQLINTLCSSKWD
PQCVIHLANMFRDIPLSGEELQFVVEKVLRMFSKLDLQEIPPLVYQLLLLSAKGSKKTVLEGIISFFNQLDKRQKEEQRVPQSADLEVATVPLDQLRHVE
GTVILHIVSAINLDQDIGEELIKHLKTEQQKDPGKALCPFSVSLLLSTAVKHRLQEQIFDFLKTSITRSCKDLQILQASKFLQDLCPQQYDVTAVILEVV
KNSAFGWDHVTQGLVDLGFSLMESYEPKKSFGGKAAETNLGLSKMPAQQACKLGASILLETFKVHEPIRSDILEQVLNRVLTKAASPVSHFIDLLSNIVV
SAPLVLQNSSSRVTETFDNLSFLPIDTVQGLLRAVQPLLKVSMSVRDSLILVLQKAIFSRQLDARKAAVAGFLLLLRNFKILGSLTSSQCSQAIGATQVQ
ADVHACYNSAANEAFCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSIMETLLSQIKQYYLPQQDLLPPLKLEGCIMAQGDQIFLQEPLAHLL
CCIQHCLAWYKSTVHLCKGAEDEEEEEDVGFEQNFEEMLESVTRRMIKSELEDFELDKSADFSPSSGVGVKNNIYAIQVMGICEVLIEYNFKIGNFSKNK
FEDVLGLFTCYNKLSEILKEKAGKNKSTLGNRIARSFLSMGFVSTLLTALFRDNAQSHEESLAVLRSSTEFMRYAVSVALQKVQQLEEMGQTDGPDGQNP
EKMFQNLCKITRVLLWRYTSIPTAVEESGKKKGKSISLLCLEGLLRIFNTMQQLYAARIPQFLQALDITDGDAEEADINVTEKAAFQIRQFQRSLVNQLS
SAEDDFNSKETQLLITILSTLSKLLDPGSQQFLQFLTWTVKICKENALEDLSCCKGLLTLLFSLHVLYKSPVSLLRELAQDIHACLGDIDQDVEIESRSH
FAIVNVKTAAPTVCLLVLGQADKVLEEVDWLIKRLTILGSDTSEDSTQASNQTQALEKGVILQLGTLLTVFHELVQTALPAGSCVDSLLRSLSKTYAILT
SLIKHYIQACRSTSNTVPGRLEKLVKLSGSHLTPQCYSFITYVQNIHSESLSFAEEKKKKKKEDETAVVSTVMAKVLRDTKPIPNLIFAIEQYEKFLIHL
SKKSKVNLMQYMKLSTSRDFRINASMLDSVLQEQNTEDAENEPDNNQSGTAEQPDENQEPQKKRRRKK*
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGGSMAQRILQLAAEGSPERLQEALQGLTEGELGDMVTRQALRGRETAALLKGIFKGSPCSQQSGVLRRLQVYK
HCVSLVESGDLHVGKVSEIIGLLMLEARQLPGHALAELATLFVEVIKRGSLSNGKSLELFSTVLTALSNSKESLAYGKGELNGEEFKKQLINTLCSSKWD
PQCVIHLANMFRDIPLSGEELQFVVEKVLRMFSKLDLQEIPPLVYQLLLLSAKGSKKTVLEGIISFFNQLDKRQKEEQRVPQSADLEVATVPLDQLRHVE
GTVILHIVSAINLDQDIGEELIKHLKTEQQKDPGKALCPFSVSLLLSTAVKHRLQEQIFDFLKTSITRSCKDLQILQASKFLQDLCPQQYDVTAVILEVV
KNSAFGWDHVTQGLVDLGFSLMESYEPKKSFGGKAAETNLGLSKMPAQQACKLGASILLETFKVHEPIRSDILEQVLNRVLTKAASPVSHFIDLLSNIVV
SAPLVLQNSSSRVTETFDNLSFLPIDTVQGLLRAVQPLLKVSMSVRDSLILVLQKAIFSRQLDARKAAVAGFLLLLRNFKILGSLTSSQCSQAIGATQVQ
ADVHACYNSAANEAFCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSIMETLLSQIKQYYLPQQDLLPPLKLEGCIMAQGDQIFLQEPLAHLL
CCIQHCLAWYKSTVHLCKGAEDEEEEEDVGFEQNFEEMLESVTRRMIKSELEDFELDKSADFSPSSGVGVKNNIYAIQVMGICEVLIEYNFKIGNFSKNK
FEDVLGLFTCYNKLSEILKEKAGKNKSTLGNRIARSFLSMGFVSTLLTALFRDNAQSHEESLAVLRSSTEFMRYAVSVALQKVQQLEEMGQTDGPDGQNP
EKMFQNLCKITRVLLWRYTSIPTAVEESGKKKGKSISLLCLEGLLRIFNTMQQLYAARIPQFLQALDITDGDAEEADINVTEKAAFQIRQFQRSLVNQLS
SAEDDFNSKETQLLITILSTLSKLLDPGSQQFLQFLTWTVKICKENALEDLSCCKGLLTLLFSLHVLYKSPVSLLRELAQDIHACLGDIDQDVEIESRSH
FAIVNVKTAAPTVCLLVLGQADKVLEEVDWLIKRLTILGSDTSEDSTQASNQTQALEKGVILQLGTLLTVFHELVQTALPAGSCVDSLLRSLSKTYAILT
SLIKHYIQACRSTSNTVPGRLEKLVKLSGSHLTPQCYSFITYVQNIHSESLSFAEEKKKKKKEDETAVVSTVMAKVLRDTKPIPNLIFAIEQYEKFLIHL
SKKSKVNLMQYMKLSTSRDFRINASMLDSVLQEQNTEDAENEPDNNQSGTAEQPDENQEPQKKRRRKK*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Parental: pGEX6P-1-HALO (DU24133)