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Synonyms
ABP-KELCH, MAV, MAYVEN
Plasmid
pKN7 KLHL2 KO sense B
Parent Plasmid
pKN7
Expressed
KLHL2 KO sense B guide
DU Number
DU48888
Genbank
ENSG00000109466
Species
Human
Vector Type
Mammalian
Antibiotic
Kan
Comments
CRISPR guide plasmid. Targets sense strand in exon 5, designed to KO KLHL2 when co-transfected with antisense guide 48896. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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