This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pSc-B-(GGSG X2) SENP1 (aa415-602)-(GGSG)-eGFP-SV40-SENP1 C603A (aa415-644)
Parent Plasmid
pSC-B
DU Number
DU24772
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCGGTGGTTCTGGTGGTGGTTCTGGTGATAGTGAAGATGAATTTCC
TGAAATTACAGAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATG
GGAATCAGGATGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGC
AAAGATATTCAAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCAT
CAATTTCTACATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGC
CAAGTGTGCATGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCT
GGTTATCAGGCAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGT
TGACATTCTTTTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTG
TTGTGGACTTTAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGG
ATAAACAATGAAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAG
CATTGACAAGAAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCA
GCAAGAAAAGCCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGGTGGT
TCTGGTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC
TGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCT
GACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGC
ACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACC
ATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTT
CGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA
AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGC
CACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAA
CTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC
AACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAA
GCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
TCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCATAA
TCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCA
CACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAA
CTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAA
ATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCC
AAACTCATCAATGTATCTTAGATAGTGAAGATGAATTTCCTGAAATTACA
GAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATGGGAATCAGGA
TGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGCAAAGATATTC
AAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCATCAATTTCTAC
ATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCA
TGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGG
CAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTT
TTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTT
TAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGGATAAACAATG
AAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAG
AAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAG
CCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGCTGGGATGTTTGCCT
GCAAATATGCTGACTGTATTACCAAAGACAGACCAATCAACTTCACACAG
CAACACATGCCATACTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCG
AAAACTCTTGTAGGTCGAC
TGAAATTACAGAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATG
GGAATCAGGATGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGC
AAAGATATTCAAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCAT
CAATTTCTACATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGC
CAAGTGTGCATGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCT
GGTTATCAGGCAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGT
TGACATTCTTTTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTG
TTGTGGACTTTAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGG
ATAAACAATGAAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAG
CATTGACAAGAAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCA
GCAAGAAAAGCCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGGTGGT
TCTGGTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC
TGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCT
GACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGC
ACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACC
ATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTT
CGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA
AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGC
CACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAA
CTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC
AACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAA
GCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
TCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCATAA
TCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCA
CACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAA
CTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAA
ATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCC
AAACTCATCAATGTATCTTAGATAGTGAAGATGAATTTCCTGAAATTACA
GAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATGGGAATCAGGA
TGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGCAAAGATATTC
AAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCATCAATTTCTAC
ATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCA
TGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGG
CAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTT
TTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTT
TAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGGATAAACAATG
AAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAG
AAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAG
CCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGCTGGGATGTTTGCCT
GCAAATATGCTGACTGTATTACCAAAGACAGACCAATCAACTTCACACAG
CAACACATGCCATACTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCG
AAAACTCTTGTAGGTCGAC
Vector Type
Other
Antibiotic
Amp; Kan
Comments
cloned overlap PCR product - SENP1 fragment with 5' and 3' linkers fused to eGFP-SV40 sequence. C603A SENP1 fragment inserted into HindIII-Sal1 sites. 33bp of pSc-B sequence between nt1570-1601 removed with NW3414/3415. Entire SENP-eGFP-SV40-SENP C603A cassette can be excised with BamHI-Sal1.
100042 R 1000 1001 1047 19007
Download datasheet