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Plasmid
pSc-B-(GGSG X2) SENP1 (aa415-602)-(GGSG)-eGFP-SV40-SENP1 (aa415-644)
Parent Plasmid
pSC-B
DU Number
DU24759
Species
Human
Sequence of Insert
View Sequence
AAGCTTGATAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAA
AGAAATAAAGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTG
AAGCATTTCGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCAT
CTGAATTGGCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGAT
GGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCT
TTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGG
ACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCA
CCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATA
TTACCTATTACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATA
CTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTT
TGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTC
AGCAGATGAATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGAC
TGTATTACCAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATA
CTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTAGG
TCGAC
AGAAATAAAGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTG
AAGCATTTCGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCAT
CTGAATTGGCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGAT
GGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCT
TTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGG
ACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCA
CCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATA
TTACCTATTACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATA
CTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTT
TGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTC
AGCAGATGAATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGAC
TGTATTACCAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATA
CTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTAGG
TCGAC
Vector Type
Other
Antibiotic
Amp; Kan
Comments
cloned overlap PCR product - SENP1 fragment with 5' and 3' linkers fused to eGFP-SV40 sequence. WT SENP1 fragment then inserted into HindIII-Sal1 sites. 33bp of pSc-B sequence exists between nt1570-1601 (NW3414/3415 to remove). Entire SENP-eGFP-SV40-SENP cassette can be excised with BamHI-Sal1.
100042 R 1000 1001 1047 19007
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