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Plasmid
pcDNA FRT TO BirA R118G UBP15 C298A HA
Parent Plasmid
pcDNA FRT TO BirA-MCS-HA
Expressed
BirA R118G-UBP15 C298A-HA
DU Number
DU47584
Genbank
Q9Y4E8
Tag
HA
BirA
Sequence of Insert
View Sequence
CTCGACATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTCTGACATCGCGACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGTACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGCTTTGACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACCCATTGATAACTCTGGACTTCTCAAAGATGGTGATGCCCAGTCACTTAAGGAACACCTTATTGATGAATTGGATTACATACTGTTGCCAACTGAAGGTTGGAATAAACTTGTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGCACGAAAGGTGGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTATATCTCACAGAATTGAAGCTATGTGAAAATGGAAACATGAATAATGTTGTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATTGAAAAGGAAATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGAACAAATACATGAGTAACACATTTGAACCACTGAATAAACCAGACAGCACCATTCAGGATGCTGGTTTATACCAAGGACAGGTATTAGTGATAGAACAGAAAAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAAGTCCCCAGGTGCATCCAATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCAAATAATTATAACAACATGAACAACAGAAATGTGAAAAACTCAAATTACTGTCTTCCATCATATACCGCTTATAAGAACTATGATTATTCGGAACCTGGAAGAAACAATGAACAGCCAGGCCTCTGTGGCCTAAGTAACTTGGGAAATACGGCTTTCATGAACTCAGCTATTCAGTGTTTGAGCAACACACCTCCACTTACTGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAGCAAATGTGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGACACAGGTAGGACGTTTTGCACCTCAGTTCTCTGGATATCAGCAGCAAGACTGTCAAGAACTGTTAGCTTTCCTATTAGATGGATTACATGAGGATTTGAATAGAATTAGGAAAAAACCATATATACAATTAAAAGATGCAGATGGAAGGCCAGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGAAATGATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTTTGTCCTGAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTTGACACTTCCATTGCCCATGAAAAAAGAACGCACCTTGGAAGTTTACTTAGTTAGAATGGATCCACTTACCAAACCTATGCAGTACAAAGTGGTTGTCCCCAAAATTGGAAACATATTAGATCTTTGTACAGCATTGTCTGCTTTGTCAGGAATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGATTTCACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGATGATATTTATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCACGTGATTATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCGAGTTATACCCACCATACTGGTTCTTCACTTTTTGGTCAGCCCTTTCTTATGGCTGTACCACGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGAGAATGTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGATCCCTACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCAAATGGCATACATGAAGAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGAATCCAGCCAGGATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTGAAGATTCAGTTGGAGGAGATAATGATTCTGAAAATGGATTATGTACTGAGGATACTTGCAAAGGTCAACTCACGGGACACAAAAAACGATTGTTTACATTCCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGATGATACCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATCTTTTCTTGCTTTGGATTGGGATCCTGATTTGAAAAAAAGATATTTTGATGAAAATGCTGCCGAGGACTTTGAAAAACATGAAAGTGTGGAGTATAAACCTCCTAAAAAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACAAAAGAAAAGCTAGGTGCTGAAGATCCCTGGTATTGTCCGAATTGTAAAGAACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCTCCAGTACTTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAGTTGGATACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATTCTTAATTAATCCAAATGCAGGTCCTTGCCGCTATAATCTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACACTATACTGCTTTTGCAAAAAATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCACTGCATCTGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTCTACCAGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGATAGCAATGATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTAACGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKGSPGEFASLDMAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNMNNRNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTAFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSALSGIPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEININRTEDTEHVIIPVCLREKFRHSSYTHHTGSSLFGQPFLMAVPRNNTEDKLYNLLLLRMCRYVKISTETEETEGSLHCCKDQNINGNGPNGIHEEGSPSEMETDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQLTGHKKRLFTFQFNNLGNTDINYIKDDTRHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDIENENCMHTNAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
Comments
silent change T2301C
100042 R 1000 1001 1047 19007
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