USP33 | Ubiquitylation Resource

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Synonyms

VDU1

Plasmid

pcDNA5D FRT/TO BirA R118G-USP33 C194A-HA (codon optimised seq)

Parent Plasmid

pcDNA5D FRT/TO BirA R118G

Expressed

BirA R118G-USP33 C194A-HA

DU Number

DU24736

Genbank

NM_201626.2 GI:391738247 with insertion of residues RFVVSCVP after K551 (present in many other published sequences for USP33)

Tag

BirA

Species

Human

Sequence of Insert

View Sequence

GGATCCATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGT
GTTACCTCATTTTGAAAGTCTTGGGAAACAGGAAAAAATTCCTAACAAAA
TGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATA
ACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAA
AGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATG
TTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAG
ACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTA
TGCTTGCAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTT
CATTGCCTCATGTAAGACAACCTCACCAAATACAAGAAAACAGTGTCCAG
GATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGT
ATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCA
GAGGTCTTACAGGTTTGAAAAATATAGGAAATACTgcTTACATGAATGCA
GCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGA
TTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTT
ATCTCAAACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTT
GTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTCG
GGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATGGATT
TGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCG
CAAACCATAACCACTGAGGAGACAATGGAAGAAGACAAGAGCCAGTCGGA
TGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAA
ATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATG
TTAATTCAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAA
AGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAATTTG
ATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAA
ACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGT
CCATTCGAATGACCTGTCTACACCACAGATCCTTCCATCAAATGAAGGTG
TTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGCAATTTGTGGCCA
GGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAA
AAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAA
CAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACC
CTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCT
TGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGAT
CATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCTTTTTTCATGGAA
TATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCC
AGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAAC
TAAAAGGTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAAT
GGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCA
CCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCC
ATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAG
GATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCAGTCATTTGCCA
TCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATC
TAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCA
GAATCTACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAG
CAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACA
TAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAAT
AAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCT
TTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTATATTGAAGACC
TGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTAT
GGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAAT
TGAGGCGGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTA
TTCGGGTAAAAAAGGCGGCCGC

Amino Acid Sequence

View Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMTGSNSHITILTLKVLPHFESLGKQEK
IPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLEN
RCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKL
GTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEED
ELRARGLTGLKNIGNTAYMNAALQALSNCPPLTQFFLDCGGLARTDKKPA
ICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLR
CLMDLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNS
DRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNS
EGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQILP
SNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISD
IFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSI
VKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFF
ARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFST
KISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAY
CRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRIS
NLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAG
YIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTE
LEIFIRVKKAAAYPYDVPDYA*

Vector Type

Mammalian

Antibiotic

Amp

Comments

49108 parental. Silent mutation at Ile190 compared to NCBI ref

100042 R 1000 1001 1047 19007

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