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Plasmid
pcDNA5-FRT/TO-KLHL13-GFP
Parent Plasmid
pcDNA FRT TO C-GFP
Expressed
KLHL13-GFP
DU Number
DU24730
Genbank
NM_033495.3
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
agatctATGCCATTGAAATGGAAAACGAGCTCTCCTGCTATCTGGAAATT
CCCAGTTCCTGTGCTTAAAACATCCAGGTCAACTCCACTTTCTCCAGCAT
ACATATCTCTCGTGGAAGAGGAAGACCAACACATGAAATTGTCCCTTGGA
GGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGCAGGACC
TACACGCATCTTTACCAGCAATACCCACAGTTCTGTGGTGTTACAGGGCT
TTGACCAGCTTCGACTTGAAGGATTGCTTTGTGATGTGACCCTGATGCCA
GGTGACACAGATGATGCTTTCCCTGTGCATAGAGTCATGATGGCATCTGC
TAGTGATTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATT
TAATGTGCATTAAACTTCATGGTGTGAGCAAAGTCGGTCTAAGGAAAATT
ATTGATTTCATTTATACTGCAAAGCTTTCTCTTAATATGGACAACCTTCA
AGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTTTGGACT
TCTGTAAAGTGTTTCTCATATCTGGGGTCACTTTAGACAACTGTGTTGAA
GTTGGACGGATTGCCAACACCTACAATCTAACCGAAGTGGATAAATACGT
TAACAGTTTCGTCTTGAAGAATTTTCCTGCATTGCTGAGCACAGGGGAGT
TCTTGAAACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGC
CTTAAGCACTGTACTGAACTTGAGCTCTTTAAGGCTACCTGTCGTTGGCT
TCGCCTGGAAGAGCCTCGGATGGACTTTGCTGCAAAATTAATGAAGAACA
TACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTGCAAACG
GTGGATTTCATGAGAACTGACAATACTTGTGTGAATTTGCTTTTGGAAGC
CAGCAATTACCAAATGATGCCATATATGCAGCCAGTTATGCAGTCAGACA
GGACTGCCATTAGGTCTGACACCACTCACTTGGTTACACTAGGAGGAGTG
CTGAGGCAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAA
GGCCCATGAGTGGAAATCGTTAGCCCCCATGGATGCCCCAAGGTACCAGC
ATGGCATCGCCGTCATTGGAAATTTTCTCTATGTGGTTGGCGGACAGAGT
AATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAGATTTGA
TCCTCGATACAATAAATGGATGCAAGTTGCATCTTTAAATGAAAAGCGCA
CCTTCTTCCACCTAAGTGCCCTCAAAGGATATCTGTATGCAGTTGGTGGG
CGAAATGCAGCAGGTGAACTGCCCACAGTAGAATGTTACAATCCAAGAAC
AAATGAATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATG
CTGGAACTGTGTATGGAGGAGTGATGTATATTTCAGGAGGAATTACTCAT
GATACTTTCCAAAAGGAGCTCATGTGCTTTGACCCTGATACTGACAAATG
GATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCATGTGTA
CAGTGGGAGAAAGGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACA
AGTGATTATGATGATGTCCTAAGCTGTGAATACTATTCACCTATCCTTGA
CCAGTGGACCCCAATTGCTGCCATGTTAAGAGGGCAGAGTGATGTTGGGG
TCGCTGTCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAAT
AATCGTTGTATGGTAGAGATAGTGCAGAAATATGATCCAGATAAAGATGA
ATGGCATAAGGTTTTTGATCTGCCAGAATCCCTTGGTGGCATTCGTGCTT
GCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCTTCTAGA
GAGTCCCCTCTTTCTGCACCTgcggccgAAGGGCGAATTCCACATTGGTCGCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGC
CCCAGTTCCTGTGCTTAAAACATCCAGGTCAACTCCACTTTCTCCAGCAT
ACATATCTCTCGTGGAAGAGGAAGACCAACACATGAAATTGTCCCTTGGA
GGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGCAGGACC
TACACGCATCTTTACCAGCAATACCCACAGTTCTGTGGTGTTACAGGGCT
TTGACCAGCTTCGACTTGAAGGATTGCTTTGTGATGTGACCCTGATGCCA
GGTGACACAGATGATGCTTTCCCTGTGCATAGAGTCATGATGGCATCTGC
TAGTGATTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATT
TAATGTGCATTAAACTTCATGGTGTGAGCAAAGTCGGTCTAAGGAAAATT
ATTGATTTCATTTATACTGCAAAGCTTTCTCTTAATATGGACAACCTTCA
AGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTTTGGACT
TCTGTAAAGTGTTTCTCATATCTGGGGTCACTTTAGACAACTGTGTTGAA
GTTGGACGGATTGCCAACACCTACAATCTAACCGAAGTGGATAAATACGT
TAACAGTTTCGTCTTGAAGAATTTTCCTGCATTGCTGAGCACAGGGGAGT
TCTTGAAACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGC
CTTAAGCACTGTACTGAACTTGAGCTCTTTAAGGCTACCTGTCGTTGGCT
TCGCCTGGAAGAGCCTCGGATGGACTTTGCTGCAAAATTAATGAAGAACA
TACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTGCAAACG
GTGGATTTCATGAGAACTGACAATACTTGTGTGAATTTGCTTTTGGAAGC
CAGCAATTACCAAATGATGCCATATATGCAGCCAGTTATGCAGTCAGACA
GGACTGCCATTAGGTCTGACACCACTCACTTGGTTACACTAGGAGGAGTG
CTGAGGCAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAA
GGCCCATGAGTGGAAATCGTTAGCCCCCATGGATGCCCCAAGGTACCAGC
ATGGCATCGCCGTCATTGGAAATTTTCTCTATGTGGTTGGCGGACAGAGT
AATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAGATTTGA
TCCTCGATACAATAAATGGATGCAAGTTGCATCTTTAAATGAAAAGCGCA
CCTTCTTCCACCTAAGTGCCCTCAAAGGATATCTGTATGCAGTTGGTGGG
CGAAATGCAGCAGGTGAACTGCCCACAGTAGAATGTTACAATCCAAGAAC
AAATGAATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATG
CTGGAACTGTGTATGGAGGAGTGATGTATATTTCAGGAGGAATTACTCAT
GATACTTTCCAAAAGGAGCTCATGTGCTTTGACCCTGATACTGACAAATG
GATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCATGTGTA
CAGTGGGAGAAAGGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACA
AGTGATTATGATGATGTCCTAAGCTGTGAATACTATTCACCTATCCTTGA
CCAGTGGACCCCAATTGCTGCCATGTTAAGAGGGCAGAGTGATGTTGGGG
TCGCTGTCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAAT
AATCGTTGTATGGTAGAGATAGTGCAGAAATATGATCCAGATAAAGATGA
ATGGCATAAGGTTTTTGATCTGCCAGAATCCCTTGGTGGCATTCGTGCTT
GCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCTTCTAGA
GAGTCCCCTCTTTCTGCACCTgcggccgAAGGGCGAATTCCACATTGGTCGCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGC
Amino Acid Sequence
View Sequence
MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYISLVEEEDQHMKLSLGGS
EMGLSSHLQSSKAGPTRIFTSNTHSSVVLQGFDQLRLEGLLCDVTLMPGD
TDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGVSKVGLRKIID
FIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVG
RIANTYNLTEVDKYVNSFVLKNFPALLSTGEFLKLPFERLAFVLSSNSLK
HCTELELFKATCRWLRLEEPRMDFAAKLMKNIRFPLMTPQELINYVQTVD
FMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLR
QQLVVSKELRMYDEKAHEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNY
DTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGYLYAVGGRN
AAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVMYISGGITHDT
FQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSD
YDDVLSCEYYSPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNR
CMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSPSRES
PLSAPAAEGRIPHWSLQPGGSTSSRAAAMVSKGEELFTGVVPILVELDGD
VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCF
SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNI
EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYKSGL*
EMGLSSHLQSSKAGPTRIFTSNTHSSVVLQGFDQLRLEGLLCDVTLMPGD
TDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGVSKVGLRKIID
FIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVG
RIANTYNLTEVDKYVNSFVLKNFPALLSTGEFLKLPFERLAFVLSSNSLK
HCTELELFKATCRWLRLEEPRMDFAAKLMKNIRFPLMTPQELINYVQTVD
FMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLR
QQLVVSKELRMYDEKAHEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNY
DTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGYLYAVGGRN
AAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVMYISGGITHDT
FQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSD
YDDVLSCEYYSPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNR
CMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSPSRES
PLSAPAAEGRIPHWSLQPGGSTSSRAAAMVSKGEELFTGVVPILVELDGD
VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCF
SRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNI
EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYKSGL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
parental 41574
100042 R 1000 1001 1047 19007
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