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Synonyms
UBCH3, UBC3, UBE2R1, E2-CDC34, cell division cycle 34
Plasmid
pET28a CDC34 C191S C223S
Parent Plasmid
pET28a original
Expressed
His-CDC34 C191S C223S
DU Number
DU24667
Genbank
P49427 NM_004359 NP_004350 Gene ID 997
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGA
GCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGG
TGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGGGCCCCCC
AACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCAT
CGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGC
ACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCG
CCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCC
CACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACG
AGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGG
AAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCG
GAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGG
TGCCCACCACGCTGGCCGAGTACTCCGTGAAGACCAAGGCGCCGGCGCCC
GACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGT
GGAGGAGGAGGCCGACAGCTCCTTCGGGGACGATGAGGATGACTCTGGCA
CGGAGGAGTCCTGAGCGGCCGC
GCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGG
TGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGGGCCCCCC
AACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCAT
CGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGC
ACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCG
CCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCC
CACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACG
AGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGG
AAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCG
GAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGG
TGCCCACCACGCTGGCCGAGTACTCCGTGAAGACCAAGGCGCCGGCGCCC
GACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGT
GGAGGAGGAGGCCGACAGCTCCTTCGGGGACGATGAGGATGACTCTGGCA
CGGAGGAGTCCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSARPLVPSSQKALLLEL
KGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPID
YPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPT
QNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRK
QVLGTKVDAERDGVKVPTTLAEYSVKTKAPAPDEGSDLFYDDYYEDGEVE
EEADSSFGDDEDDSGTEES*
KGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPID
YPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPT
QNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRK
QVLGTKVDAERDGVKVPTTLAEYSVKTKAPAPDEGSDLFYDDYYEDGEVE
EEADSSFGDDEDDSGTEES*
Vector Type
Bacterial
Antibiotic
Amp
Comments
native methionine absent. Ala35 of fusion protein = Ala2 of NP_004350
100042 R 1000 1001 1047 19007
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