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Plasmid
pGEX6P-1-ISG15 1-157 C78S
Parent Plasmid
pGEX6P1
Expressed
GST ISG15 1-157 C78S
DU Number
DU24665
Genbank
NP_005092
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATT
CCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGA
TCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCAC
CCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCT
GGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATCCGACGAACCTC
TGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTG
CGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGA
GGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGG
AGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTG
TTCATGAATCTGCGCCTGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGC
TAAACAGAGCCTGGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGCTAAA
CAGAGCCTGGCGGGCGGAGCTAAGCGGCCGC
CCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGA
TCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCAC
CCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCT
GGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATCCGACGAACCTC
TGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTG
CGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGA
GGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGG
AGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTG
TTCATGAATCTGCGCCTGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGC
TAAACAGAGCCTGGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGCTAAA
CAGAGCCTGGCGGGCGGAGCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGWDLTVKMLAGNEFQVSL
SSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGS
TVLLVVDKSDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQD
DLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGWDLTVKMLAGNEFQVSL
SSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGS
TVLLVVDKSDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQD
DLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
3' Not1 site 100bp downstream of stop codon
100042 R 1000 1001 1047 19007
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