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Plasmid
pMK-RQ USP30 Cter GFP donor
Parent Plasmid
pMK-RQ
DU Number
DU48549
Genbank
NM_032663.3
Species
Human
Sequence of Insert
View Sequence
GTCCAAAATGGAAATAACTTTTAAATTAAAAATTTTTAAAGTACATAAAATTTACATATGCATATGTATGTGTGTATATATGTGTGTATGTATAAAATATAAAGAAAATTTAAAATTTCTTGGGATACCATCTAGAGATAGCCAATTAAACACTTAGGGTCATGCACTCTATAAAGTTGTACATCACACATACATATAGTGTACATAAACACATACACTCATTGATACAAACATCCACATATACACAATATACATATACTCATAGAATGTACATCTTGCCATGCCAGTAAATACAGATCTGTATTTTAATAGTTGTGTAGCATTTGGTGGTATGGACTTACCATTGTATTCATCCCCTATTTAACATTAGCATTCTTTTGTTTTTGCCTATAAAGTCTTTTTGTTAAAATTGTAAACCCCTGACATGTGTTCGTATCATTCAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCtTaCAaGAGGTCtTGagtTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTagtcGtATGCAGCAtCAGtctCAGGAGTGCAAGTCTGAAGAAgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagTGACTGTGCCCTCCTGCAAGGCTAGAGCTGATGGCACTGTCTGCACTGTCCAGGAAAAAAGTAAAACTGTACTGTTGCGTGTGCAAGCGGCCCCACTAGAGCCTTCCAGCCTTCTGGTGTGTTCTAAGAGCAGGCTCCACCTGGGAGCCAGCCCCAGTTCACACCAAACCAGGCTCCCTGAACAGTCCTGTTCATGTGTGTAGGTGGTTCTGTTGTGTTAAGAAAGCATTCATTATGTCCGGAGTGTCTTTTTACTCATCTGATACAGGTAATTAAAAGAACTCAGATTCTTGAAGCCACCGTTTTCATATTGTAATGTTAGGTGTTCTCAGAGGGGAGGTACCTTTGTCTAATCAACGTTTCCACTTAGATCTTTTATTTTTAATAAGCAGGCCCATAAAAATTGTTGACAAGAATTAATGAAATTATTAAAGGCAACAATTTAGAAGAAAAAGTGCCTTTCACTTTCGATTGCTTTTGTAGCACGTCCATTGTGAAATATTCCTTCCAGGCTACTCAAAGGATAGCAAGAGAACAGGTAAATGATGCCTAAAGAACACC
Vector Type
Mammalian
Antibiotic
Kan
Comments
CRISPR donor plasmid designed to tag USP30 (longest variants) with GFP at the C-terminus by an in-frame insertion upstream of the stop codon in exon 13. To be used in co-transfected with 2 guide RNAs DU48612 and DU48521 and should function with either wild-type or D10A nickase Cas9. Sequence of insert shows the donor including flanking homology arms, several silent mutations are present to prevent recognition and attack of the donor by Cas9 during the recombination procedure.
100042 R 1000 1001 1047 19007
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