SAE2 | Ubiquitylation Resource

This reagent and more can be found at our sister site MRC PPU Reagents & Services

Request Now

Plasmid

pETDuet-1 GST Thrombin SAE1, 6-His Precission SAE 2

Parent Plasmid

pETDuet1

Expressed

6His-SAE2

DU Number

DU20972

Genbank

NM_005499.2

Tag

6His

Species

Human

Sequence of Insert

View Sequence

CCATGGGACACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCA
ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGG
GGGCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCA
AGAATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGAT
ACTATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACA
TGTTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTT
ACCCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGAC
TATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGA
TAACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATG
TTCCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACT
ATCAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCA
GAGAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCAGAACCTATAC
ATTGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAA
GATGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTG
GGAACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTG
ACATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGAT
CCAGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTT
GACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACT
GGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAG
AATGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAG
CTATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAG
CAGAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCT
GCAATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAG
TATGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTA
TTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTG
GAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTT
TTTGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCAC
TGGATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTG
ACTGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAA
GATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAG
ATGGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCT
AATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCT
TCAAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTC
ATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCC
CCGGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAAC
CAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGC
AAGATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAAT
GCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAA
AGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTG
ATGATGTCATAGCATTAGATTGAGTCGAC

Amino Acid Sequence

View Sequence

MGHHHHHHLEVLFQGPMALSRGLPRELAEAVAGGRVLVVGAGGIGCELLK
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial dual expression for Sae1 and 2. Sae2 was subcloned first into pET-Duet6P-1 MCS1 to give SC20936, and then Sae1 was subcloned into MCS2. Estimated protein MW of protein Sae2 : 73147 Daltons

100042 R 1000 1001 1047 19007

Download datasheet