SAE1 | Ubiquitylation Resource

Synonyms

SUMO-1 Activating Enzyme E1 N Subunit

Plasmid

pETDuet-1 GST Thrombin SAE1, 6-His Precission SAE 2

Parent Plasmid

pETDuet1

Expressed

GST-SAE1

DU Number

DU20972

Genbank

NM_005500.2

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGTACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCA
ACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATT
TGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTG
GGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATT
AACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGT
TGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCG
GTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTT
TGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAA
TGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTA
ACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACAT
GGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAAC
GTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTAT
ATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCA
TCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCATGGTGGAGAAGGAGG
AGGCTGGCGGCGGCATTAGCGAGGAGGAGGCGGCACAGTATGACCGGCAG
ATCCGCCTGTGGGGACTGGAGGCCCAGAAACGGCTGCGGGCCTCTCGGGT
GCTTCTTGTCGGCTTGAAAGGACTTGGGGCTGAAATTGCCAAGAATCTCA
TCTTGGCAGGAGTGAAAGGACTGACCATGCTGGATCACGAACAGGTAACT
CCAGAAGATCCCGGAGCTCAGTTCTTGATTCGTACTGGGTCTGTTGGCCG
AAATAGGGCTGAAGCCTCTTTGGAGCGAGCTCAGAATCTCAACCCCATGG
TGGATGTGAAGGTGGACACTGAGGATATAGAGAAGAAACCAGAGTCATTT
TTCACTCAATTCGATGCTGTGTGTCTGACTTGCTGCTCCAGGGATGTCAT
AGTTAAAGTTGACCAGATCTGTCACAAAAATAGCATCAAGTTCTTTACAG
GAGATGTTTTTGGCTACCATGGATACACATTTGCCAATCTAGGAGAGCAT
GAGTTTGTAGAGGAGAAAACTAAAGTTGCCAAAGTTAGCCAAGGAGTAGA
AGATGGGCCCGACACCAAGAGAGCAAAACTTGATTCTTCTGAGACAACGA
TGGTCAAAAAGAAGGTGGTCTTCTGCCCTGTTAAAGAAGCCCTGGAGGTG
GACTGGAGCAGTGAGAAAGCAAAGGCTGCTCTGAAGCGCACGACCTCCGA
CTACTTTCTCCTTCAAGTGCTCTTAAAGTTCCGTACAGATAAAGGAAGAG
ATCCCAGTTCTGATACATATGAGGAAGATTCTGAGTTGTTGCTCCAGATA
CGAAATGATGTGCTTGACTCACTGGGTATTAGTCCTGACCTGCTTCCTGA
GGACTTTGTCAGGTACTGCTTCTCCGAGATGGCCCCAGTGTGTGCGGTGG
TTGGAGGGATTTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAGCGGGAC
CCTCCTCACAACAACTTCTTCTTCTTCGATGGCATGAAGGGGAATGGGAT
TGTGGAGTGCCTTGGCCCCAAGTGACTCGAG

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMVEKEEAGGGISEEEAAQYDRQIR
LWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPE
DPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFT
QFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEF
VEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDW
SSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRN
DVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPP
HNNFFFFDGMKGNGIVECLGPK*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial dual expression for Sae1 and 2. Sae2 was subcloned first into pET-Duet6P-1 MCS1 to give SC20936, and then Sae1 was subcloned into MCS2
Estimated protein MW of protein Sae1 : 64733.10 Daltons

100042 R 1000 1001 1047 19007