USP30

Expresses USP30 BspHI KO guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets. Target site situated on sense strand in the first common coding exon 2 and guide designed to KO BspHI by introducing an Indel via CRISPR. To be screened for by PCR and BspHI digestion, loss of the site shall indicate a deletion event and potential frameshift.