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Plasmid
pU6D USP30 Cter antisense guide
Parent Plasmid
pU6D gRNA
Expressed
USP30 Cter antisense guide
DU Number
DU48521
Genbank
NM_032663.3
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
Expresses USP30 Cter KI antisense guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra G added to improve expression from U6 promoter). Targets just upstream of the stop codon, PAM site situated on antisense strand. Designed to KI an in-frame GFP via CRISPR (wild-type or Nickase Cas9) when used in conjunction with the sense guide DU48612 and the GFP donor DU48549. To be screened for by GFP analysis and verified by PCR and western.
100042 R 1000 1001 1047 19007
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