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Plasmid
pU6D USP30 ScaI KO guide
Parent Plasmid
pU6D gRNA
Expressed
USP30 ScaI KO guide
DU Number
DU48519
Genbank
NM_032663.3
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
Expresses USP30 ScaI KO guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets. Target site situated on sense strand of exon 3 and guide designed to KO ScaI by introducing an Indel via CRISPR. To be screened for by PCR and ScaI digestion, loss of the site shall indicate a deletion event and potential frameshift.
100042 R 1000 1001 1047 19007
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