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Plasmid
pET28a 6His-TEV Xenopus l. Cullin 1 1-412 V367R L371D
Parent Plasmid
pET28a original
Expressed
6His-TEV Xenopus l. Cullin 1 1-412 V367R L371D
DU Number
DU49867
Genbank
NP_001089364.1 with three silent changes
Tag
6His
Species
Xenopus laevis
Sequence of Insert
View Sequence
TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCCTCCCACCACCATCATCACCACGAAAATCTGTACTTCCAAGGTGGCTCTATGAGCAGCAACCGTAGCCAGAACCCGCATGGCCTGAAACAGATCGGTCTGGACCAGATCTGGGATGATCTGCGTGCGGGCATTCAGCAGGTGTACACCCGTCAGTCCATGGCTAAGTCTCGTTACATGGAGCTGTATACGCATGTGTACAACTACTGCACCTCTGTTCATCAATCCAACCAGGCTCGTGGCGCGGGTGTTCCGCCGAGCAAAAGCAAGAAGGGCCAGACCCCGGGCGGCGCGCAGTTCGTTGGTCTGGAACTGTACAAACGTCTGAAAGAATTCCTGAAAAATTACCTGACGAACCTGCTGAAAGATGGTGAAGACCTGATGGATGAATCCGTACTGAAATTCTATACTCAACAGTGGGAAGATTACCGTTTCTCCAGCAAAGTTCTGAACGGTATCTGCGCCTATCTGAACCGTCACTGGGTACGTCGTGAATGTGATGAAGGCCGCAAGGGTATCTACGAAATCTATTCCCTGGCGCTTGTTACCTGGCGTGACTGCCTGTTCAAGCCGCTGAACAAACAAGTTACCAACGCCGTACTGAAACTGATTGAAAAAGAACGCAACGGCGAGACCATCAACACTCGTCTGATTAGCGGTGTAGTGCAGAGCTACGTCGAACTGGGTCTGAACGAAGACGATGCGTTCGCTAAAGGCCCGACCCTGACTGTTTACAAAGAAAGCTTCGAAAGCCAGTTCCTGGCTGACACCGAACGTTTCTACACCCGTGAATCTACCGAATTTCTGCAGCAGAACCCGGTGACCGAATATATGAAAAAAGCCGAAGCGCGCCTGCTGGAAGAACAGCGTCGCGTTCAGGTTTATCTGCACGAATCCACTCAGGACGAGCTGGCGCGTAAATGTGAACAAGTTCTGATTGAAAAACACCTGGAAATCTTCCACACGGAGTTCCAAAACCTGCTGGACGCTGACAAAAACGAAGACCTGGGTCGCATGTATAACCTGGTGTCCCGCATCCAGGATGGCCTGGGTGAACTGAAGAAACTGCTGGAAACTCACATTCATAACCAGGGTCTGGCGGCAATCGAGAAGTGCGGCGAAGCAGCACAGAACGACCCGAAAATGTATCGCCAGACGGTAGATGATGTGCACAAAAAGTATAACGCACTGGTAATGTCCGCTTTCAACAACGATGCGGGCTTCGTCGCAGCACTGGACAAGGCGTGCGGTCGCTTCATTAACAACAACGCGGTGACCAAAATGGCCTAAGAATTCGAGCTCCGTCGAC
Amino Acid Sequence
View Sequence
MGSSHHHHHHENLYFQGGSMSSNRSQNPHGLKQIGLDQIWDDLRAGIQQV
YTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGA
QFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSK
VLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTN
AVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKES
FESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHE
STQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQD
GLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYRQTVDDVHKKYNALV
MSAFNNDAGFVAALDKACGRFINNNAVTKMA*
YTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGA
QFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSK
VLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTN
AVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKES
FESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHE
STQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQD
GLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYRQTVDDVHKKYNALV
MSAFNNDAGFVAALDKACGRFINNNAVTKMA*
Vector Type
Bacterial
Antibiotic
Kan
100042 R 1000 1001 1047 19007
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