PIAS1 | Ubiquitylation Resource

Synonyms

protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik

Plasmid

pcDNA5-FRT/TO-EYFP-PIAS1 (siRNA resistant) deletion SAP

Parent Plasmid

pcDNA5 FRT/TO

Expressed

EYFP-PIAS1 (siRNA resistant) delta Ala5-Arg64

DU Number

DU24568

Genbank

BAC35902 Gene ID: 56469

Tag

YFP

Species

Mouse

Sequence of Insert

View Sequence

ggatccATGGCGGACAGTTTCCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACTTATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGATGAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCGGGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGTCGTCCTATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATACCATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAGGGAATAAGAAACCCAGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTTCGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTACTGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAACCAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGACAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTCCTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTTCCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGATACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCCACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGCAGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCCCGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGCACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCTTGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCAGGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATCTCATTGGACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSFPQKI
MTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLLPVSLLGPKHE
LELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCF
AFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNL
CVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVS
WTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTAD
PDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKK
PTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSK
KEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVIDLTIDSSSD
EEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTPSLPAVDTSYI
NTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVS
DDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSN
SLRESHGHGVASRSSADTASIFGIIPDIISLD*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Silent mutations at S247 R248 P249 (agc cga cca → agt cgt cct ) and R303 N304 P305 (agg aat ccg → aga aac cca) plus SAP mutation (delat Ala5-Arg64)

100042 R 1000 1001 1047 19007