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PIAS1

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Synonyms
protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik
Plasmid
pcDNA5-FRT/TO-EYFP-PIAS1 siRNA resistant
Parent Plasmid
pcDNA5 FRT/TO
Expressed
EYFP-PIAS1 siRNA resistant
DU Number
DU24546
Genbank
BAC35902 Gene ID: 56469
Tag
YFP
Species
Mouse
Sequence of Insert
ggatccATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTC
TGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACGGACGCA
AACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGCTGTAGT
CCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTTCCCTCA
GAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTC
CTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACTTATGAT
GGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAA
ACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACC
CGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGATGAACTG
ATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCGGGAAAC
CTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCT
CCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTA
AGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACC
CAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACC
TTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTagtcgtcctATT
AATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATACCATTGT
TGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAGTATATC
TTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAG
GGAATAagaaacccaGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAAC
TGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTTCGCTGC
TGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACC
TGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGA
GAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATG
AACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTACTGCACA
GACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAG
GTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATG
GTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAACCAGTCC
TCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGACAGCTC
GTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTCCTTCCC
TGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTTCCTCAT
CAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGATACAAG
CTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCCACATGA
CGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCA
GGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGCAGCGGC
GGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCCCGTATA
CCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGCACATCT
CTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCTTGTGTC
TTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCAGGAGCA
GCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATCTCATTG
GACTGAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSAELKQ
MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKE
LYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLL
PVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASD
NSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSC
PQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLS
TTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSR
ALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDA
TLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKE
DGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEV
IDLTIDSSSDEEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTP
SLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTS
LLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSS
GSNSSLVSSNSLRESHGHGVASRSSADTASIFGIIPDIISLD*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Silent mutations at S247 R248 P249 (agc cga cca → agt cgt cct ) and R303 N304 P305 (agg aat ccg → aga aac cca)
100042 R 1000 1001 1047 19007
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