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Plasmid
pET15b 6HIS SUMO FANCL L7A D78R L79A I182V (chicken)
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO FANCL L7A D78R L79A I182V (chicken)
DU Number
DU25683
Genbank
NM_001033941.1
Tag
6His
SUMO
Species
Chicken
Sequence of Insert
View Sequence
ATGGCGGGTCGGGGATCTGCACTCCGCCAGTGTCCGCTGCTGCTGCCTCAGAACCGCGAGGGGACGGCCTATGAGGGCTTCGTCAGCGCGCAGGGTAGAGACTTCCACATCCGGATACTGTTACCAACAGATTCTCAACTGAAAAATGCAAGGATAGAGTGTAGCTGGCACCTAAAGAAGATACTGCATGGATATCAGCATATTTTGAAACAGAGGTTGCACAGTTGTCCAAGGGCAGTCAGTTTTGTGGTGGAGCTGAAAACTATTTTGGAAATTGCTTTGAAGAATACACAAGAGCTGCATGTACCGTGGCCACCAGAATACTACTCCTGTCTCATCAGAGATCTAGAAATATTGGGTTGGAATAAAGTTGCTTATGTGGATACTGGTCTCACCACAGTCAAGCTAAAAGCTGAAGACTCTCGTGGTCGACAACATCTGATCACTCTGAAGTTAAATGCAAAGTATCCTACTGAACCACCAGACTGCGTTGTGGATTTTCCTGTTCAGTTTGCCATTTCTTGGATGCCACAGGACTCATTAGTAGACATCCATAATCAGTTCTTGGCAGCATTAGAATCGCTGAAGGAGTTCTGGGACACCATGGATGAAATTGATGGGAAGACGTGGGTACTGGAGCCAGAAAATCCCGCAAGGTGTGCTACTTCAAGAAGGATCGCCATAGGGAACAACGTTTCCGTGAACATAGAGGTAGATCCCAGGCATCCAAAGATGCTCCCTGAGTGTTACTTCCTTGGAGCAGATCATGCGGTTAACCCTTTGAGAACTAAGCTGAACAATAACATGCACTTGTGGGATCCAGAAATTAGTTTATTACAAAACTTGAAAGACCTCTTAGAAATTGATTTTCCATCTCGTGCTGCATTAGAAAAAAGTGACTTTACTAAGGACTGTGGAATCTGCTATGCCTACCGCCTCAATGGCACCACGCCAGATCAAGTGTGCAATGAGCCCCGATGCGGACAACCTTTTCATCAGGCTTGCCTGTATGAGTGGCTACAAGGTCTTCCTTCCAGCAGACAGAGCTTTAATGTCATCTTTGGTGAATGTCCATATTGCAACAAGCCACTGACAGTGAAATCTTCCATGAAGAAACCTTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMAGRGSALRQCPLLLPQNREGTAYEGFVSAQGRDFHIRILLPTDSQLKNARIECSWHLKKILHGYQHILKQRLHSCPRAVSFVVELKTILEIALKNTQELHVPWPPEYYSCLIRDLEILGWNKVAYVDTGLTTVKLKAEDSRGRQHLITLKLNAKYPTEPPDCVVDFPVQFAISWMPQDSLVDIHNQFLAALESLKEFWDTMDEIDGKTWVLEPENPARCATSRRIAIGNNVSVNIEVDPRHPKMLPECYFLGADHAVNPLRTKLNNNMHLWDPEISLLQNLKDLLEIDFPSRAALEKSDFTKDCGICYAYRLNGTTPDQVCNEPRCGQPFHQACLYEWLQGLPSSRQSFNVIFGECPYCNKPLTVKSSMKKP*
Vector Type
Bacterial
Antibiotic
Amp
Comments
linker after SENP cleavage deleted. Has I182V SNP change but it is a tolerated change. Silent mutation A191
100042 R 1000 1001 1047 19007
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