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Plasmid
pcDNA5-FRT/TO-EYFP-PIAS4
Parent Plasmid
pcDNA5 FRT/TO
Expressed
EYFP-PIAS4
DU Number
DU24517
Genbank
BC029874 NM_015897.2
Tag
YFP
Species
Human
Sequence of Insert
View Sequence
ggatccAAGATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAG
TTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTA
AGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAG
TTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGAC
CCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCC
TGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCC
AGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAA
GTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAG
TCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAG
CCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGTG
CATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGG
AACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTAC
TCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGT
GAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATA
AGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCAC
CTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAA
CTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCT
CATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAG
CTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGA
GATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGA
TGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGC
TTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGAT
GTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACG
GGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAG
TACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAG
CTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATG
GGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACG
GGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGA
TGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGG
AGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGGGGCCCCGGCCCAAG
CGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGACCCCGGCC
GCACACTCGACgcggccgc
TTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTA
AGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAG
TTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGAC
CCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCC
TGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCC
AGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAA
GTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAG
TCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAG
CCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGTG
CATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGG
AACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTAC
TCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGT
GAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATA
AGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCAC
CTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAA
CTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCT
CATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAG
CTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGA
GATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGA
TGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGC
TTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGAT
GTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACG
GGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAG
TACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAG
CTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATG
GGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACG
GGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGA
TGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGG
AGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGGGGCCCCGGCCCAAG
CGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGACCCCGGCC
GCACACTCGACgcggccgc
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVP
AC*
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVP
AC*
Vector Type
Mammalian
Antibiotic
Amp
Comments
constructed by Anne Seifert (Ron Hay Grp). Clone harbours an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.
100042 R 1000 1001 1047 19007
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