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PIAS1

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Synonyms
protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik
Plasmid
pcDNA5-FRT/TO-EYFP-PIAS1
Parent Plasmid
pcDNA5 FRT/TO
Expressed
EYFP-PIAS1
DU Number
DU24516
Genbank
BAC35902 Gene ID: 56469
Tag
YFP
Species
Mouse
Sequence of Insert
ggatccATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAG
AGTTTCTGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACG
GACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGC
TGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTT
CCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATT
CAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACT
TATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGG
ACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAG
TCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGAT
GAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCG
GGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCA
GCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTC
CAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTT
CCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAG
GTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGA
CCAATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATAC
CATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAG
TATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGA
GCAAAGGGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAA
GTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTT
CGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCA
CTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAAT
GAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCC
CATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTAC
TGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCC
AATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAG
TTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAAC
CAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGA
CAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTC
CTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTT
CCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGA
TACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCC
ACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTC
TTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGC
AGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCC
CGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGC
ACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCT
TGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCA
GGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATC
TCATTGGACTGAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSAELKQ
MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKE
LYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLL
PVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASD
NSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSC
PQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLS
TTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSR
ALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDA
TLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKE
DGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEV
IDLTIDSSSDEEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTP
SLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTS
LLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSS
GSNSSLVSSNSLRESHGHGVASRSSADTASIFGIIPDIISLD*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Constructed by Anne Seifert (Ron Hay Grp)
100042 R 1000 1001 1047 19007
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