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Plasmid
pU6 DCUN1D5 gRNA B
Parent Plasmid
pU6 gRNA BamHI
Expressed
DCUN1D5 gRNA B
DU Number
DU48398
Genbank
ENSG00000137692
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
Expresses DCUN1D5 guide RNA B, 19mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra G added to aid expression). Targets exon 2, PAM site is situated on the sense strand. To be used in conjunction with DCUN1D5 guide RNA A, a ssDNA KO donor and Cas9 D10A nickase.
100042 R 1000 1001 1047 19007
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