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Synonyms
CUL1
Plasmid
pMA CUL1 Nter FLAG donor
Parent Plasmid
pMA
DU Number
DU48351
Genbank
NM_003592.2
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
TGCCCTTTTTTCTTTGAGTTTGTCTTTCTAATTGTCATCTAGGTCAAGTGATATATATTTTTGATAACTTCCGAAGGCAAAGGAAGGCAACATAGTTGTGCTTGTGCAAACTTTTACCCAGGTTTTCTCTTGAGTGCTGATATCATAATGATTTTGAACTAAAATTCTAAGCGTATTTTTGTACACAGACCATATGGTTAACATGAATGTGTTTGCACATAGAAAGAGAAATGGTCTCTAGAGTGGGCCCTGCAGACTGAGTTTTTCTGTCAGTGTGTCTCACCTTATTACAGTACCCCAGAGACAATCCACTGATTCTCTTTATTTCTTCCTCAGGTTTGCCTGCAATGAGATTTCATTCTCTACATTTAAAGGACATCCTTTCTGAGCTGCTGTGAATAAATTTGGAATGGTACTGTATATTTTCATCTAATGGAGAACTAGCTGTACTTTGAATAAGGATTGCTGCACTGGACGACTTTAGAACATCCCTCACAATGaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgccGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAGACTACAAGGACGATGACGATAAGTCGTCAACCCGGAGCCAGAACCCCCACGGCCTGAAGCAaATcGGtttaGAtCAaATaTGGGACGACCTCAGAGCCGGCATCCAGCAGGTGTACACACGGCAGAGCATGGCCAAGTCCAGATATATGGAGCTCTACACGTATCCTCCTGCCTAGCGCAGGTTGATTGCTTAGAGTTTTGATTATTTACTAGTATGACTTATGAATTATTAGAATTTTATATTGTTTTCTCCTCCCAGTTCATCATGTAAAATAATCGCAGGGTTCATTTTTAGCCTTAAGTTTTTGAACAGAGTAGAACTGGTTCATTTTTAGTCAAATGCAGCACTGGGCAGTACAGTCACCACTGGGCACATGTGGCTGTTTACATTTGAAATATTTTAAAGTTCCTTAGGCACACTAGCTGTGTGATTAATAAATAAAACATTTCTGTCATGTAGAAGGTTCCGTTGGGCAAAGCTGGTCTAAAGCAAGGTCCTGAGGTTCTCAGCAGATCATTTGTTTTACGTCCATTTGTTTATATATTGTCTATGACTGCCTTGTGTTATGGGAACAGAGTT
Vector Type
Mammalian
Antibiotic
Amp
Comments
Designed to insert an in-frame Puro-2A-FLAG into the first common coding exon (exon 2 isoform 1). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will n-terminally tag CUL1 with FLAG, to be used in conjunction with DU48275.
100042 R 1000 1001 1047 19007
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