USP30 | Ubiquitylation Resource

Plasmid

pGEX USP30 57-end C77A

Parent Plasmid

pGEX6P1

Expressed

GST-3C-USP30 57-end C77A

DU Number

DU48346

Genbank

NM_032663.3

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCATGACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCTGGCCTTGTTAATTTAGGGAACACCGCCTTCATGAACTCCCTGCTACAAGGCCTGTCTGCCTGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCCAGGGATCAGAAGGAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTTGCACCTTCTGAAAGCCTTGTCCTGCCAAGAAGTTACTGATGATGAGGTCTTAGATGCAAGCTGCTTGTTGGATGTCTTAAGAATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTCATTACCTCGTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTGATGTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAAATTACCTGCCGCACAAGAGGGTCACCTCACCCCACATCCAATCACTGGAAGTCTCAACATCCTTTTCATGGAAGACTCACTAGTAATATGGTCTGCAAACACTGTGAACACCAGAGTCCTGTTCGATTTGATACCTTTGATAGCCTTTCACTAAGTATTCCAGCCGCCACATGGGGTCACCCATTGACCCTGGACCACTGCCTTCACCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTACAAAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAGAGGACCACTTTTGTTAAACAGTTAAAACTAGGGAAGCTCCCTCAGTGTCTCTGCATCCACCTACAGCGGCTGAGCTGGTCCAGCCACGGCACGCCTCTGAAGCGGCATGAGCACGTGCAGTTCAATGAGTTCCTGATGATGGACATTTACAAGTACCACCTCCTTGGACATAAACCTAGTCAACACAACCCTAAACTGAACAAGAACCCAGGGCCTACACTGGAGCTGCAGGATGGGCCGGGAGCCCCCACACCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAATGGCGCCTGCTCCCCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTTCCCTCTCCCAGTTGTTCCCGACTACAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGGAGGTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTTCCAGGATGCAGCACCAGAGCCAGGAGTGCAAGTCTGAAGAATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMTERKKRRKGLVPGLVNL
GNTAFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHL
LKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSL
EDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHP
FHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHF
ISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCI
HLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKN
PGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPL
PVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQW
LWVSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE*

Vector Type

Bacterial

Antibiotic

Amp

Comments

57-end, C77A. Single silent nucleotide variation, T>C at 471 bp.

100042 R 1000 1001 1047 19007