Rap80/Cezanne | Ubiquitylation Resource

Synonyms

UIMC1; X2HRIP110; ZA20D1; OTUD7B

Plasmid

pcDNA5D FRT/TO GFP Rap80 60-124-NLS-Cezanne OTU (OTUD7B 124-438) C194A

Parent Plasmid

pcDNA5D FRT/TO GFP

Expressed

GFP Rap80 60-124-NLS-Cezanne OTU (OTUD7B 124-438) C194A

DU Number

DU49553

Genbank

Rap80: NM_016290.4, Cezanne: NM_020205.3

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

GGATCCACGAAGACAAAACAGTCGAATAGAGCAAAGTGTTTGGCCAAAAGAAAAATCGCACAGATGACAGAAGAAGAACAGTTTGCTCTGGCTCTCAAAATGAGTGAGCAGGAAGCTAGGGAGGTGAACAGCCAGGAGGAGGAAGAAGAGGAGCTCTTGAGGAAAGCCATTGCTGAAAGCCTGAATAGTTGCCGGCCTTCTCCACCGAAGAAGAAGCGCAAGGTCAATGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGACCCCACCTCTCAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACGCTCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAACTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGTGGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTTGTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTCAAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATTCCACTGTCCTCTTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSTKTKQS
NRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIA
ESLNSCRPSPPKKKRKVNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLI
EQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNALLHAASLGMWGF
HDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQK
EWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVL
RRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQ
AHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDS
DNVRLASVILSLEVKLHLLHSYMNVKWIPLSS*

Vector Type

Mammalian

Antibiotic

Amp

Comments

(with silent changes to kill internal BamHI sites)

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