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Plasmid
pGEX TEV HHARI (D183-S230) - HOIP (C744-K1072)
Parent Plasmid
pGEX TEV
Expressed
GST-TEV-HHARI (D183-S230) - HOIP (C744-K1072)
DU Number
DU24314
Genbank
NM_017999.4 (HOIP) and NP_005735 (HHARI)
Tag
GST
Species
Human
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGEFDMPCQICYLNYPNSYFTGLEC
GHKFCMQCWSEYLTTKIMEEGMGQTISCPACGRPDLTDDTQLLSYFSTLD
IQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEAT
CPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQE
NGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNC
RVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPG
GGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLD
PATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQS
IPRRRK*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGEFDMPCQICYLNYPNSYFTGLEC
GHKFCMQCWSEYLTTKIMEEGMGQTISCPACGRPDLTDDTQLLSYFSTLD
IQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEAT
CPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQE
NGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNC
RVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPG
GGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLD
PATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQS
IPRRRK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
(DU20757) parental. Two rounds of insertion mutagenesis with NW2676/77 and NW2678/79 to replace HOIP 697-743 (QEC....TDMV) in DU22629 with HHARI 183-230 (DMPC...QTIS). mutations in precursor corrected with NW2730/31
100042 R 1000 1001 1047 19007
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