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Plasmid
pGEX TEV RNF144 (L17-S64) - HOIP (C744-K1072)
Parent Plasmid
pGEX TEV
Expressed
GST-TEV-RNF144 (L17-S64) - HOIP (C744-K1072)
DU Number
DU24298
Genbank
NM_017999.4 (HOIP) and NM_014746.3 (RNF144)
Tag
GST
Species
Human
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSPEFLVSCKLCLGEYPVEQMTT
IAQCQCIFCTLCLKQYVELLIKEGLETAISCPACGRPDLTDDTQLLSYFS
TLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQL
EATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMY
LQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPE
PNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARA
VPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAH
SLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPL
GQSIPRRRK*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSPEFLVSCKLCLGEYPVEQMTT
IAQCQCIFCTLCLKQYVELLIKEGLETAISCPACGRPDLTDDTQLLSYFS
TLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQL
EATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMY
LQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPE
PNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARA
VPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAH
SLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPL
GQSIPRRRK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
(DU20757) parental. Two rounds of insertion mutagenesis with NW2680/81 and NW2682/83 to replace HOIP 697-743 (QEC....TDMV) in DU22629 with RNF144A 17-64 (LVS....AIS)
100042 R 1000 1001 1047 19007
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