VAPB | Ubiquitylation Resource

Synonyms

VAP-B , ALS8

Plasmid

pET28a- VAPB aa1-210 P56S -TEV-HALO (C ter)

Parent Plasmid

pET28a original

Expressed

VAPB aa1-210 P56S- HALO

DU Number

DU24174

Genbank

NM_004738.3

Tag

HALO

Species

Human

Sequence of Insert

View Sequence

ccATGGGTCGCGGATCCATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAG
CCGCAGCACGAGCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCAC
CAACCTAAAGCTTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGA
AGACTACAGCACCACGTAGGTACTGTGTGAGGTCCAACAGCGGAATCATC
GATGCAGGGGCCTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTA
TGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGTTTG
CTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCG
GAAGACCTTATGGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGA
GAATGATAAACCACATGATGTAGAAATAAATAAAATTATATCCACAACTG
CATCAAAGACAGAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTG
GATGACACCGAAGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGG
TGAAGTTCAGAGGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATG
GACTGCGGATGAGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCAGAA
TTCGAGCTCCGTCGACaagcttccGAAAACCTGTATTTTCAGGGCatggc
agaaatcggtactggctttccattcgacccccattatgtggaagtcctgg
gcgagcgcatgcactacgtcgatgttggtccgcgcgatggcacccctgtg
ctgttcctgcacggtaacccgacctcctcctacgtgtggcgcaacatcat
cccgcatgttgcaccgacccatcgctgcattgctccagacctgatcggta
tgggcaaatccgacaaaccagacctgggttatttcttcgacgaccacgtc
cgcttcatggatgccttcatcgaagccctgggtctggaagaggtcgtcct
ggtcattcacgactggggctccgctctgggtttccactgggccaagcgca
atccagagcgcgtcaaaggtattgcatttatggagttcatccgccctatc
ccgacctgggacgaatggccagaatttgcccgcgagaccttccaggcctt
ccgcaccaccgacgtcggccgcaagctgatcatcgatcagaacgttttta
tcgagggtacgctgccgatgggtgtcgtccgcccgctgactgaagtcgag
atggaccattaccgcgagccgttcctgaatcctgttgaccgcgagccact
gtggcgcttcccaaacgagctgccaatcgccggtgagccagcgaacatcg
tcgcgctggtcgaagaatacatggactggctgcaccagtcccctgtcccg
aagctgctgttctggggcaccccaggcgttctgatcccaccggccgaagc
cgctcgcctggccaaaagcctgcctaactgcaaggctgtggacatcggcc
cgggtctgaatctgctgcaagaagacaacccggacctgatcggcagcgag
atcgcgcgctggctgtcgacgctcgagatttccggcTGAgcggccgc

Amino Acid Sequence

View Sequence

MGRGSMAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVK
TTAPRRYCVRSNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFA
PTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTA
SKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDG
LRMRKTVQSNSPISAEFELRRQASENLYFQGMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISG*

Vector Type

Bacterial

Antibiotic

Kan

Comments

5 additional amino acids (MGRGS) on N-terminus prior to native start

100042 R 1000 1001 1047 19007