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Plasmid
pet28a 6HIS GFP TEV USP35
Parent Plasmid
pET28a 6His GFP TEV
Expressed
6HIS GFP TEV USP35
DU Number
DU46088
Genbank
Q9P2H5
Tag
GFP
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGT CAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGC CGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTC TACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCT GCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCG CGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCC CGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCC TGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCG TGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTG GCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTG CCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCG AGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTC CTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAA AGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCG CCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTT GTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGAC TGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATA TTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAG TTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTC TAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGT ACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTC CCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTC GGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGT TCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATC AAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCA GGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGC TCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAAC ACATGCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTT CAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGA CCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCC ATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCC TGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGC TGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAG TCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTC TGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCT GCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGAT GCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTG CACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATC ACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAA GGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAG AAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAA AGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGA AGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGG AAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCC GGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGT TAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACT GCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGC CAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCT GCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGC TGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGC AGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTG CTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCG ATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTG TCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAA GGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCG AGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAG GACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCA GGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACAT CTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGC TCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAG ACTGGTCTTCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
YNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGP
VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLE
NLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALL
ALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGG
AGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACA
QVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQA
QQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPL
ELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKG
LAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHE
AFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYE
PVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIG
LINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLE
HSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRI
CQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEA
FTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRR
QRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEE
VGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHP
DAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAE
KVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRG
QAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYL
FNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRT
EPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDED
KDEDEGSPGGCNPAGGNGGDFHRLVF*
TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
YNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGP
VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLE
NLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALL
ALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGG
AGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACA
QVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQA
QQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPL
ELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKG
LAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHE
AFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYE
PVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIG
LINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLE
HSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRI
CQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEA
FTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRR
QRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEE
VGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHP
DAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAE
KVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRG
QAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYL
FNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRT
EPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDED
KDEDEGSPGGCNPAGGNGGDFHRLVF*
Vector Type
Bacterial
Antibiotic
Kan
100042 R 1000 1001 1047 19007
Download datasheet
Parental 25159